Effect of peptidases secreted by the opportunistic pathogen Scedosporium aurantiacum on human epithelial cells

Zhiping Han*, Liisa Kautto, Wieland Meyer, Sharon C.-A. Chen, Helena Nevalainen

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Peptidases secreted by a clinical high-virulence Scedosporium aurantiacum isolate (strain WM 06.482; CBS 136046) under normoxic and hypoxic conditions were separated via size-exclusion chromatography, and peptidase activities present in each fraction were determined using class-specific substrates. The fractions demonstrating peptidase activity were assessed for their effects on the attachment and viability of A549 human lung epithelial cells in vitro. Of the peptidases detected in the size-exclusion chromatography fractions, the elastase-like peptidase reduced cell viability, the chymotrypsin-like peptidase was associated with cell detachment, and the cysteine peptidases were able to abolish both cell attachment and viability. The loss of cell viability and attachment became more prominent with an increase in the peptidase activity and could also be specifically prevented by addition of class-specific peptidase inhibitors. Our findings indicate that peptidases secreted by S. aurantiacum can breach the human alveolar epithelial cell barrier and, thus, may have a role in the pathobiology of the organism.

Original languageEnglish
Pages (from-to)814-822
Number of pages9
JournalCanadian Journal of Microbiology
Volume65
Issue number11
DOIs
Publication statusPublished - Nov 2019

Keywords

  • Scedosporium aurantiacum
  • Peptidases
  • Human alveolar epithelial cells
  • Cell attachment
  • Cell viability

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