Effect of phospholipid deposits on adhesion of bacteria to contact lenses

Negar Babaei Omali*, Nicholas Proschogo, Hua Zhu, Zhenjun Zhao, Jennie Diec, Roya Borazjani, Mark D P Willcox

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

PURPOSE.: Protein and lipid deposits on contact lenses may contribute to clinical complications. This study examined the effect of phospholipids on the adhesion of bacteria to contact lenses. METHODS.: Worn balafilcon A (n = 11) and senofilcon A (n = 11) were collected after daily wear and phospholipids were extracted in chloroform:methanol. The amount of phospholipid was measured by electrospray ionization mass spectrometry. Unworn lenses soaked in phospholipids were exposed to Pseudomonas aeruginosa and Staphylococcus aureus. After 18 h incubation, the numbers of P. aeruginosa or S. aureus that adhered to the lenses were measured. Phospholipid was tested for possible effects on bacterial growth. RESULTS.: A broad range of sphingomyelins (SM) and phosphatidylcholines (PC) were detected from both types of worn lenses. SM (16:0) (m/z 703) and PC (34:2) (m/z 758) were the major phospholipids detected in the lens extracts. Phospholipids did not alter the adhesion of any strain of P. aeruginosa or S. aureus (p > 0.05). Phospholipids (0.1 mg/mL) showed no effect on the growth of P. aeruginosa 6294 or S. aureus 031. CONCLUSIONS.: Phospholipids adsorb/absorb to contact lenses during wear, however, the major types of phospholipids adsorbed to lenses do not alter bacterial adhesion or growth.

Original languageEnglish
Pages (from-to)52-61
Number of pages10
JournalOptometry and Vision Science
Volume89
Issue number1
DOIs
Publication statusPublished - Jan 2012
Externally publishedYes

Keywords

  • Phospholipid
  • Pseudomonas aeruginosa
  • Silicone hydrogel contact lenses
  • Staphylococcus aureus
  • Tear film

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