TY - JOUR
T1 - Effect of prolonged gelling time on the intrinsic properties of barium alginate microcapsules and its biocompatibility
AU - Vaithilingam, Vijayaganapathy
AU - Kollarikova, Gabriella
AU - Qi, Meirigeng
AU - Lacik, Igor
AU - Oberholzer, Jose
AU - Guillemin, Gilles J.
AU - Tuch, Bernard E.
PY - 2011/9
Y1 - 2011/9
N2 - Pericapsular fibrotic overgrowth (PFO) may be attributed to an immune response against microcapsules themselves or to antigen shedding through microcapsule pores from encapsulated islet tissue. Modification of microcapsules aimed at reducing pore size should prevent PFO and improve graft survival. This study investigated the effect of increased gelling time (20 vs. 2min) in barium chloride on intrinsic properties of alginate microcapsules and tested their biocompatibility in-vivo. Prolonged gelling time affected neither permeability nor size of the microcapsules. However, prolonged gelling time for 20min produced brittle microcapsules compared to 2min during compression test. Encapsulation of human islets in both types of microcapsules affected neither islet viability nor function. The presence of PFO when transplanted into a large animal model such as baboon and its absence in small animal models such as rodents suggest that the host immune response towards alginate microcapsules is species rather than alginate specific.
AB - Pericapsular fibrotic overgrowth (PFO) may be attributed to an immune response against microcapsules themselves or to antigen shedding through microcapsule pores from encapsulated islet tissue. Modification of microcapsules aimed at reducing pore size should prevent PFO and improve graft survival. This study investigated the effect of increased gelling time (20 vs. 2min) in barium chloride on intrinsic properties of alginate microcapsules and tested their biocompatibility in-vivo. Prolonged gelling time affected neither permeability nor size of the microcapsules. However, prolonged gelling time for 20min produced brittle microcapsules compared to 2min during compression test. Encapsulation of human islets in both types of microcapsules affected neither islet viability nor function. The presence of PFO when transplanted into a large animal model such as baboon and its absence in small animal models such as rodents suggest that the host immune response towards alginate microcapsules is species rather than alginate specific.
UR - http://www.scopus.com/inward/record.url?scp=80051730205&partnerID=8YFLogxK
U2 - 10.3109/02652048.2011.586067
DO - 10.3109/02652048.2011.586067
M3 - Article
C2 - 21827357
AN - SCOPUS:80051730205
SN - 0265-2048
VL - 28
SP - 499
EP - 507
JO - Journal of Microencapsulation
JF - Journal of Microencapsulation
IS - 6
ER -