Effects of natural radiation, photosynthetically active radiation and artificial ultraviolet radiation-b on the chloroplast organization and metabolism of porphyra acanthophora var. brasiliensis (rhodophyta, bangiales)

Zenilda L. Bouzon, Fungyi Chow, Carmen S. Zitta, Rodrigo W. Dos Santos, Luciane C. Ouriques, Marthiellen R. Marthiellen, Luz K P Osorio, Claudiane Gouveia, Roberta De Paula Martins, Alexandra Latini, Fernanda Ramlov, Marcelo Maraschin, Eder C. Schmidt*

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We undertook a study of Porphyra acanthophora var. brasiliensis to determine its responses under ambient conditions, photosynthetically active radiation (PAR), and PAR+UVBR (ultraviolet radiation-B) treatment, focusing on changes in ultrastructure, and cytochemistry. Accordingly, control ambient samples were collected in the field, and two different treatments were performed in the laboratory. Plants were exposed to PAR at 60 μmol photons m -2 s-1 and PAR + UVBR at 0.35 W m-2 for 3 h per day during 21 days of in vitro cultivation. Confocal laser scanning microscopy analysis of the vegetative cells showed single stellate chloroplast in ambient and PAR samples, but in PAR+UVBR-exposed plants, the chloroplast showed alterations in the number and form of arms. Under PAR+UVBR treatment, the thylakoids of the chloroplasts were disrupted, and an increase in the number of plastoglobuli was observed, in addition to mitochondria, which appeared with irregular, disrupted morphology compared to ambient and PAR samples. After UVBR exposure, the formation of carpospores was also observed. Plants under ambient conditions, as well as those treated with PAR and PAR+UVBR, all showed different concentrations of enzymatic response, including glutathione peroxidase and reductase activity. In summary, the present study demonstrates that P. acanthophora var. brasiliensis shows the activation of distinct mechanisms against natural radiation, PAR and PAR+UVBR.

Original languageEnglish
Pages (from-to)1467-1479
Number of pages13
JournalMicroscopy and Microanalysis
Volume18
Issue number6
DOIs
Publication statusPublished - Dec 2012
Externally publishedYes

Keywords

  • carpospores
  • chloroplasts
  • enzymatic response
  • photosynthetic pigments
  • Porphyra acanthophora var. brasiliensis
  • ultraviolet radiation-B

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