Effects of Phyllanthus plant extracts on duck hepatitis B virus in vitro and in vivo

Andrew Shead; Karen Vickery; Aniko Pajkos; Robert Medhurst; John Freiman; Robert Dixon; Yvonne Cossart

doi:10.1016/0166-3542(92)90033-2

Effects of Phyllanthus plant extracts on duck hepatitis B virus in vitro and in vivo

Andrew Shead^*, Karen Vickery, Aniko Pajkos, Robert Medhurst, John Freiman, Robert Dixon, Yvonne Cossart

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

The effects of extracts of five Australian Phyllanthus species (P. hirtellus, P. gunnii, P. gasstroemii, P. similis and P. tenellus), other plant extracts and the antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. All 5 Phyllanthus species caused 50% inhibition at concentrations of dry weight between 350-800 μg/ml, which is comparable with the effect described for P. amarus on the DNAp of human and woodchuck hepatitis B viruses. Incubation of P. hirtellus with 100 ID₅₀ DHBV neutralized infection. However, neither P. gasstroemi extract, given by intraperitoneal injection (i.p.) at a dose of 20 mg/kg 3 times per week to ducklings early in the incubation period, or P. hirtellus extract, given to established DHBV carrier ducklings, prevented or eliminated infection.

Original language	English
Pages (from-to)	127-138
Number of pages	12
Journal	Antiviral Research
Volume	18
Issue number	2
DOIs	https://doi.org/10.1016/0166-3542(92)90033-2
Publication status	Published - 1992
Externally published	Yes

Keywords

DNA polymerase
Duck hepatitis B
Neutralization
Phyllanthus

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10.1016/0166-3542(92)90033-2

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title = "Effects of Phyllanthus plant extracts on duck hepatitis B virus in vitro and in vivo",

abstract = "The effects of extracts of five Australian Phyllanthus species (P. hirtellus, P. gunnii, P. gasstroemii, P. similis and P. tenellus), other plant extracts and the antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. All 5 Phyllanthus species caused 50% inhibition at concentrations of dry weight between 350-800 μg/ml, which is comparable with the effect described for P. amarus on the DNAp of human and woodchuck hepatitis B viruses. Incubation of P. hirtellus with 100 ID50 DHBV neutralized infection. However, neither P. gasstroemi extract, given by intraperitoneal injection (i.p.) at a dose of 20 mg/kg 3 times per week to ducklings early in the incubation period, or P. hirtellus extract, given to established DHBV carrier ducklings, prevented or eliminated infection.",

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author = "Andrew Shead and Karen Vickery and Aniko Pajkos and Robert Medhurst and John Freiman and Robert Dixon and Yvonne Cossart",

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doi = "10.1016/0166-3542(92)90033-2",

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T1 - Effects of Phyllanthus plant extracts on duck hepatitis B virus in vitro and in vivo

AU - Shead, Andrew

AU - Vickery, Karen

AU - Pajkos, Aniko

AU - Medhurst, Robert

AU - Freiman, John

AU - Dixon, Robert

AU - Cossart, Yvonne

PY - 1992

Y1 - 1992

N2 - The effects of extracts of five Australian Phyllanthus species (P. hirtellus, P. gunnii, P. gasstroemii, P. similis and P. tenellus), other plant extracts and the antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. All 5 Phyllanthus species caused 50% inhibition at concentrations of dry weight between 350-800 μg/ml, which is comparable with the effect described for P. amarus on the DNAp of human and woodchuck hepatitis B viruses. Incubation of P. hirtellus with 100 ID50 DHBV neutralized infection. However, neither P. gasstroemi extract, given by intraperitoneal injection (i.p.) at a dose of 20 mg/kg 3 times per week to ducklings early in the incubation period, or P. hirtellus extract, given to established DHBV carrier ducklings, prevented or eliminated infection.

AB - The effects of extracts of five Australian Phyllanthus species (P. hirtellus, P. gunnii, P. gasstroemii, P. similis and P. tenellus), other plant extracts and the antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. All 5 Phyllanthus species caused 50% inhibition at concentrations of dry weight between 350-800 μg/ml, which is comparable with the effect described for P. amarus on the DNAp of human and woodchuck hepatitis B viruses. Incubation of P. hirtellus with 100 ID50 DHBV neutralized infection. However, neither P. gasstroemi extract, given by intraperitoneal injection (i.p.) at a dose of 20 mg/kg 3 times per week to ducklings early in the incubation period, or P. hirtellus extract, given to established DHBV carrier ducklings, prevented or eliminated infection.

KW - DNA polymerase

KW - Duck hepatitis B

KW - Neutralization

KW - Phyllanthus

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JO - Antiviral Research

JF - Antiviral Research

IS - 2

ER -

Effects of Phyllanthus plant extracts on duck hepatitis B virus in vitro and in vivo

Abstract

Keywords

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