Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis

S. H. Phua*, P. L. Bergquist, H. E D Lane

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The gene coding for the 33,000 dalton protein (B protein) of the mini-F plasmid has been mapped by minicell polypeptide analysis to 47.3-48.2 Fkb. Transcription of the gene is initiated near 47.3 Fkb. Gene B mutants overproduce the 42,000 dalton protein (A protein) of mini-F. Insertions of Tn 5 in the B gene and in the incD region cause instability of plasmid inheritance. Mini-F plasmids delected for part of gene B are not maintained in the absence of the incD region. B protein and the incD region appear to interact in promoting mini-F maintenance.

Original languageEnglish
Pages (from-to)353-355
Number of pages3
JournalMGG Molecular & General Genetics
Volume188
Issue number2
DOIs
Publication statusPublished - Dec 1982
Externally publishedYes

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