Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis

S. H. Phua; P. L. Bergquist; H. E D Lane

doi:10.1007/BF00332700

Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis

S. H. Phua^*, P. L. Bergquist, H. E D Lane

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The gene coding for the 33,000 dalton protein (B protein) of the mini-F plasmid has been mapped by minicell polypeptide analysis to 47.3-48.2 Fkb. Transcription of the gene is initiated near 47.3 Fkb. Gene B mutants overproduce the 42,000 dalton protein (A protein) of mini-F. Insertions of Tn 5 in the B gene and in the incD region cause instability of plasmid inheritance. Mini-F plasmids delected for part of gene B are not maintained in the absence of the incD region. B protein and the incD region appear to interact in promoting mini-F maintenance.

Original language	English
Pages (from-to)	353-355
Number of pages	3
Journal	MGG Molecular & General Genetics
Volume	188
Issue number	2
DOIs	https://doi.org/10.1007/BF00332700
Publication status	Published - Dec 1982
Externally published	Yes

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title = "Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis",

abstract = "The gene coding for the 33,000 dalton protein (B protein) of the mini-F plasmid has been mapped by minicell polypeptide analysis to 47.3-48.2 Fkb. Transcription of the gene is initiated near 47.3 Fkb. Gene B mutants overproduce the 42,000 dalton protein (A protein) of mini-F. Insertions of Tn 5 in the B gene and in the incD region cause instability of plasmid inheritance. Mini-F plasmids delected for part of gene B are not maintained in the absence of the incD region. B protein and the incD region appear to interact in promoting mini-F maintenance.",

author = "Phua, {S. H.} and Bergquist, {P. L.} and Lane, {H. E D}",

year = "1982",

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doi = "10.1007/BF00332700",

language = "English",

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T1 - Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis

AU - Phua, S. H.

AU - Bergquist, P. L.

AU - Lane, H. E D

PY - 1982/12

Y1 - 1982/12

N2 - The gene coding for the 33,000 dalton protein (B protein) of the mini-F plasmid has been mapped by minicell polypeptide analysis to 47.3-48.2 Fkb. Transcription of the gene is initiated near 47.3 Fkb. Gene B mutants overproduce the 42,000 dalton protein (A protein) of mini-F. Insertions of Tn 5 in the B gene and in the incD region cause instability of plasmid inheritance. Mini-F plasmids delected for part of gene B are not maintained in the absence of the incD region. B protein and the incD region appear to interact in promoting mini-F maintenance.

AB - The gene coding for the 33,000 dalton protein (B protein) of the mini-F plasmid has been mapped by minicell polypeptide analysis to 47.3-48.2 Fkb. Transcription of the gene is initiated near 47.3 Fkb. Gene B mutants overproduce the 42,000 dalton protein (A protein) of mini-F. Insertions of Tn 5 in the B gene and in the incD region cause instability of plasmid inheritance. Mini-F plasmids delected for part of gene B are not maintained in the absence of the incD region. B protein and the incD region appear to interact in promoting mini-F maintenance.

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U2 - 10.1007/BF00332700

DO - 10.1007/BF00332700

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JO - MGG Molecular & General Genetics

JF - MGG Molecular & General Genetics

SN - 0026-8925

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ER -

Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis

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