Toe-clipping is a standard technique for marking reptiles and amphibians individually, but concerns have been raised about the impact of the practice on animal welfare, survival, and behavior. We used a long-term mark-recapture dataset to investigate the impact of toe removal on free-ranging adult Cane Toads (Rhinella marina). Our analysis of 213 toads showed no impact of the number of toes removed on growth rates for mass or snout-urostyle length, nor any effect on body condition. Trials with sub-adult toads on a laboratory raceway revealed a short-term impact of toe-clipping on willingness to move (i.e., decreased immediately post-clipping), but no other significant impacts on locomotion. In summary, toe-clipping had minimal effects on Cane Toad locomotor ability, growth rate, or body condition.