Efficiency of recombination reactions catalyzed by class 1 integron integrase IntI1

C. M. Collis, G. D. Recchia, M. J. Kim, H. W. Stokes, R. M. Hall*

*Corresponding author for this work

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The class 1 integron integrase, IntI1, recognizes two distinct types of recombination sites, attI sites, found in integrons, and members of the 59-be family, found in gene cassettes. The efficiencies of the integrative version of the three possible reactions, i.e., between two 59-be, between attI1 and a 59-be, or between two attI1 sites, were compared. Recombination events involving two attI1 sites were significantly less efficient than the reactions in which a 59-be participated, and the attI1 × 59-be reaction was generally preferred over the 59-be × 59-be reaction. Recombination of attI1 with secondary sites was less efficient than the 59-be × secondary site reaction.

Original languageEnglish
Pages (from-to)2535-2542
Number of pages8
JournalJournal of Bacteriology
Volume183
Issue number8
DOIs
Publication statusPublished - 2001

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