TY - JOUR
T1 - Efficiency of recombination reactions catalyzed by class 1 integron integrase IntI1
AU - Collis, C. M.
AU - Recchia, G. D.
AU - Kim, M. J.
AU - Stokes, H. W.
AU - Hall, R. M.
PY - 2001
Y1 - 2001
N2 - The class 1 integron integrase, IntI1, recognizes two distinct types of recombination sites, attI sites, found in integrons, and members of the 59-be family, found in gene cassettes. The efficiencies of the integrative version of the three possible reactions, i.e., between two 59-be, between attI1 and a 59-be, or between two attI1 sites, were compared. Recombination events involving two attI1 sites were significantly less efficient than the reactions in which a 59-be participated, and the attI1 × 59-be reaction was generally preferred over the 59-be × 59-be reaction. Recombination of attI1 with secondary sites was less efficient than the 59-be × secondary site reaction.
AB - The class 1 integron integrase, IntI1, recognizes two distinct types of recombination sites, attI sites, found in integrons, and members of the 59-be family, found in gene cassettes. The efficiencies of the integrative version of the three possible reactions, i.e., between two 59-be, between attI1 and a 59-be, or between two attI1 sites, were compared. Recombination events involving two attI1 sites were significantly less efficient than the reactions in which a 59-be participated, and the attI1 × 59-be reaction was generally preferred over the 59-be × 59-be reaction. Recombination of attI1 with secondary sites was less efficient than the 59-be × secondary site reaction.
UR - http://www.scopus.com/inward/record.url?scp=0035091147&partnerID=8YFLogxK
U2 - 10.1128/JB.183.8.2535-2542.2001
DO - 10.1128/JB.183.8.2535-2542.2001
M3 - Article
C2 - 11274113
AN - SCOPUS:0035091147
VL - 183
SP - 2535
EP - 2542
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 8
ER -