TY - JOUR
T1 - Efficient removal of various textile dyes from wastewater by novel thermo-halotolerant laccase
AU - Motamedi, Elaheh
AU - Kavousi, Kaveh
AU - Sadeghian Motahar, Seyedeh Fatemeh
AU - Reza Ghaffari, Mohammad
AU - Sheykh Abdollahzadeh Mamaghani, Atefeh
AU - Hosseini Salekdeh, Ghasem
AU - Ariaeenejad, Shohreh
PY - 2021/10
Y1 - 2021/10
N2 - A novel thermostable/halotolerant metagenome-derived laccase (PersiLac2) from tannery wastewater was purified to remove textile dyes in this study. The enzyme was highly active over a wide temperature and pH range and maintained 73.35% of its initial activity after 30 days, at 50 °C. The effect of various metal and organic-solvent tolerance on PersiLac2 showed, retaining greater than 53% activity at 800 mM of metal ions, 52.12% activity at 6 M NaCl, and greater than 44.09% activity at 20% organic solvents. PersiLac2 manifested effective removal of eight different textile dyes from azo, anthraquinone, and triphenylmethane families. It decolorized 500 mg/L of Alizarin yellow, Carmine, Congo red and Bromothymol blue with 99.74–55.85% efficiency after 15 min, at 50 °C, without mediator. This enzyme could practically remove dyes from a real textile effluent and it displayed significant detoxification in rice seed germination tests. In conclusion, PersiLac2 could be useful in future for decolorization/detoxification of wastewater.
AB - A novel thermostable/halotolerant metagenome-derived laccase (PersiLac2) from tannery wastewater was purified to remove textile dyes in this study. The enzyme was highly active over a wide temperature and pH range and maintained 73.35% of its initial activity after 30 days, at 50 °C. The effect of various metal and organic-solvent tolerance on PersiLac2 showed, retaining greater than 53% activity at 800 mM of metal ions, 52.12% activity at 6 M NaCl, and greater than 44.09% activity at 20% organic solvents. PersiLac2 manifested effective removal of eight different textile dyes from azo, anthraquinone, and triphenylmethane families. It decolorized 500 mg/L of Alizarin yellow, Carmine, Congo red and Bromothymol blue with 99.74–55.85% efficiency after 15 min, at 50 °C, without mediator. This enzyme could practically remove dyes from a real textile effluent and it displayed significant detoxification in rice seed germination tests. In conclusion, PersiLac2 could be useful in future for decolorization/detoxification of wastewater.
KW - Decolorization
KW - Detoxification
KW - Halotolerant
KW - Metagenome
KW - Thermostable laccase
UR - http://www.scopus.com/inward/record.url?scp=85109029207&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2021.125468
DO - 10.1016/j.biortech.2021.125468
M3 - Article
C2 - 34320748
AN - SCOPUS:85109029207
SN - 0960-8524
VL - 337
SP - 1
EP - 10
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 125468
ER -