Electron microscopy finder-grids link quantitative cytometry with high-resolution confocal imaging in the study of TGF-β1 expression in the small intestine mucosa in growing pigs

Michał M. Godlewski; Piotr Pietrzak; Anna Kotunia; Romuald Zabielski; Helena Nevalainen

doi:10.1016/j.livsci.2010.06.022

Electron microscopy finder-grids link quantitative cytometry with high-resolution confocal imaging in the study of TGF-β1 expression in the small intestine mucosa in growing pigs

Michał M. Godlewski^*, Piotr Pietrzak, Anna Kotunia, Romuald Zabielski, Helena Nevalainen

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A new method is proposed linking the high-throughput quantitative population analysis with high-resolution imaging systems. Samples from piglet small intestine labelled against transforming growth factor β1 (TGF-β1) were overlayed with electron microscopy finder-grids and scanned by SCAN^R scanning cytometer. From the tissue map generated by SCAN^R, regions of interest (ROI) with TGF-β1-positive cells were selected and their coordinates stored from the sample overview. ROI were then located under the confocal microscope enabling detail visualization of the pattern of intracellular localization of the studied cytokine.

Original language	English
Pages (from-to)	49-52
Number of pages	4
Journal	Livestock Science
Volume	133
Issue number	1-3
DOIs	https://doi.org/10.1016/j.livsci.2010.06.022
Publication status	Published - Sept 2010

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10.1016/j.livsci.2010.06.022

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title = "Electron microscopy finder-grids link quantitative cytometry with high-resolution confocal imaging in the study of TGF-β1 expression in the small intestine mucosa in growing pigs",

abstract = "A new method is proposed linking the high-throughput quantitative population analysis with high-resolution imaging systems. Samples from piglet small intestine labelled against transforming growth factor β1 (TGF-β1) were overlayed with electron microscopy finder-grids and scanned by SCAN\textasciicircum{}R scanning cytometer. From the tissue map generated by SCAN\textasciicircum{}R, regions of interest (ROI) with TGF-β1-positive cells were selected and their coordinates stored from the sample overview. ROI were then located under the confocal microscope enabling detail visualization of the pattern of intracellular localization of the studied cytokine.",

author = "Godlewski, \{Micha{\l} M.\} and Piotr Pietrzak and Anna Kotunia and Romuald Zabielski and Helena Nevalainen",

year = "2010",

month = sep,

doi = "10.1016/j.livsci.2010.06.022",

language = "English",

volume = "133",

pages = "49--52",

journal = "Livestock Science",

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Electron microscopy finder-grids link quantitative cytometry with high-resolution confocal imaging in the study of TGF-β1 expression in the small intestine mucosa in growing pigs. / Godlewski, Michał M.; Pietrzak, Piotr; Kotunia, Anna et al.
In: Livestock Science, Vol. 133, No. 1-3, 09.2010, p. 49-52.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Electron microscopy finder-grids link quantitative cytometry with high-resolution confocal imaging in the study of TGF-β1 expression in the small intestine mucosa in growing pigs

AU - Godlewski, Michał M.

AU - Pietrzak, Piotr

AU - Kotunia, Anna

AU - Zabielski, Romuald

AU - Nevalainen, Helena

PY - 2010/9

Y1 - 2010/9

N2 - A new method is proposed linking the high-throughput quantitative population analysis with high-resolution imaging systems. Samples from piglet small intestine labelled against transforming growth factor β1 (TGF-β1) were overlayed with electron microscopy finder-grids and scanned by SCAN^R scanning cytometer. From the tissue map generated by SCAN^R, regions of interest (ROI) with TGF-β1-positive cells were selected and their coordinates stored from the sample overview. ROI were then located under the confocal microscope enabling detail visualization of the pattern of intracellular localization of the studied cytokine.

AB - A new method is proposed linking the high-throughput quantitative population analysis with high-resolution imaging systems. Samples from piglet small intestine labelled against transforming growth factor β1 (TGF-β1) were overlayed with electron microscopy finder-grids and scanned by SCAN^R scanning cytometer. From the tissue map generated by SCAN^R, regions of interest (ROI) with TGF-β1-positive cells were selected and their coordinates stored from the sample overview. ROI were then located under the confocal microscope enabling detail visualization of the pattern of intracellular localization of the studied cytokine.

UR - https://www.scopus.com/pages/publications/77957118851

U2 - 10.1016/j.livsci.2010.06.022

DO - 10.1016/j.livsci.2010.06.022

M3 - Article

AN - SCOPUS:77957118851

SN - 1871-1413

VL - 133

SP - 49

EP - 52

JO - Livestock Science

JF - Livestock Science

IS - 1-3

ER -

Electron microscopy finder-grids link quantitative cytometry with high-resolution confocal imaging in the study of TGF-β1 expression in the small intestine mucosa in growing pigs

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