Electron microscopy of the K2 killer effect of Saccharomyces cerevisiae T206 on a mesophilic wine yeast

A. S. Vadasz, D. B. Jagganath, I. S. Pretorius, A. S. Gupthar

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Abstract

A mesophilic wine yeast, Saccharomyces cerevisiae CSIR Y217 K-R- was subjected to the K2 killer effect of Saccharomyces cerevisiae T206 K+R+ in a liquid grape medium. The lethal effect of the K2 mycoviral toxin was confirmed by methylene blue staining. Scanning electron microscopy of cells from challenge experiments revealed rippled cell surfaces, accompanied by cracks and pores, while those unaffected by the toxin, as in the control experiments, showed a smooth surface. Transmission electron microscopy revealed that the toxin damaged the cell wall structure and perturbed cytoplasmic membranes to a limited extent.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume78
Issue number2
DOIs
Publication statusPublished - 2000
Externally publishedYes

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