Electron microscopy of the K2 killer effect of Saccharomyces cerevisiae T206 on a mesophilic wine yeast

A. S. Vadasz; D. B. Jagganath; I. S. Pretorius; A. S. Gupthar

doi:10.1023/A:1026588220367

Electron microscopy of the K₂ killer effect of Saccharomyces cerevisiae T206 on a mesophilic wine yeast

A. S. Vadasz, D. B. Jagganath, I. S. Pretorius, A. S. Gupthar

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A mesophilic wine yeast, Saccharomyces cerevisiae CSIR Y217 K^-R^- was subjected to the K₂ killer effect of Saccharomyces cerevisiae T206 K⁺R⁺ in a liquid grape medium. The lethal effect of the K₂ mycoviral toxin was confirmed by methylene blue staining. Scanning electron microscopy of cells from challenge experiments revealed rippled cell surfaces, accompanied by cracks and pores, while those unaffected by the toxin, as in the control experiments, showed a smooth surface. Transmission electron microscopy revealed that the toxin damaged the cell wall structure and perturbed cytoplasmic membranes to a limited extent.

Original language	English
Pages (from-to)	117-122
Number of pages	6
Journal	Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume	78
Issue number	2
DOIs	https://doi.org/10.1023/A:1026588220367
Publication status	Published - 2000
Externally published	Yes

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abstract = "A mesophilic wine yeast, Saccharomyces cerevisiae CSIR Y217 K-R- was subjected to the K2 killer effect of Saccharomyces cerevisiae T206 K+R+ in a liquid grape medium. The lethal effect of the K2 mycoviral toxin was confirmed by methylene blue staining. Scanning electron microscopy of cells from challenge experiments revealed rippled cell surfaces, accompanied by cracks and pores, while those unaffected by the toxin, as in the control experiments, showed a smooth surface. Transmission electron microscopy revealed that the toxin damaged the cell wall structure and perturbed cytoplasmic membranes to a limited extent.",

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AU - Jagganath, D. B.

AU - Pretorius, I. S.

AU - Gupthar, A. S.

PY - 2000

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N2 - A mesophilic wine yeast, Saccharomyces cerevisiae CSIR Y217 K-R- was subjected to the K2 killer effect of Saccharomyces cerevisiae T206 K+R+ in a liquid grape medium. The lethal effect of the K2 mycoviral toxin was confirmed by methylene blue staining. Scanning electron microscopy of cells from challenge experiments revealed rippled cell surfaces, accompanied by cracks and pores, while those unaffected by the toxin, as in the control experiments, showed a smooth surface. Transmission electron microscopy revealed that the toxin damaged the cell wall structure and perturbed cytoplasmic membranes to a limited extent.

AB - A mesophilic wine yeast, Saccharomyces cerevisiae CSIR Y217 K-R- was subjected to the K2 killer effect of Saccharomyces cerevisiae T206 K+R+ in a liquid grape medium. The lethal effect of the K2 mycoviral toxin was confirmed by methylene blue staining. Scanning electron microscopy of cells from challenge experiments revealed rippled cell surfaces, accompanied by cracks and pores, while those unaffected by the toxin, as in the control experiments, showed a smooth surface. Transmission electron microscopy revealed that the toxin damaged the cell wall structure and perturbed cytoplasmic membranes to a limited extent.

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JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

IS - 2

ER -

Electron microscopy of the K₂ killer effect of Saccharomyces cerevisiae T206 on a mesophilic wine yeast

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