Electron paramagnetic resonance: site-directed spin labeling

Louise J. Brown*, Joanna E. Hare

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

This chapter provides an overview for performing and interpreting electron paramagnetic resonance (EPR) measurements on spin-labeled proteins. It focuses on site-directed spin labeling (SDSL) methods as appropriate for monitoring dynamics and conformational changes in membrane proteins. The chapter provides recent examples that illustrate the various SDSL-EPR techniques for revealing the connection between structure and mechanism for membrane pumps, channels, and transporters. The examples include the use of SDSL-EPR strategies for studying gating mechanisms, substrate and drug binding, and overall dynamics and structural arrangement of membrane transporters and channels accompanying their function. The chapter presents other recent reviews on SDSL in membrane proteins and other protein systems, as well as recent reviews on technological advances in methodology, instrumentation, and data analysis. It concludes with a discussion on some current challenges of SDSL for membrane proteins and the strategies under development to address these challenges.

Original languageEnglish
Title of host publicationPumps, channels and transporters
Subtitle of host publicationmethods of functional analysis
EditorsRonald J. Clarke, Mohammed A. A. Khalid
Place of PublicationNew Jersey
PublisherJohn Wiley & Sons
Pages327-365
Number of pages39
ISBN (Electronic)9781119085126
ISBN (Print)9781118858806
DOIs
Publication statusPublished - 15 Sep 2015

Publication series

NameChemical Analysis: A series of monographs on analytical chemistry and its applications
Volume183

Keywords

  • Electron paramagnetic resonance
  • Interspin distance measurements
  • Site-directed spin labeling

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