Electropermeabilization of adherent cells with cochlear implant electrical stimulation in vitro

Carrie Newbold, Andrew Farrington, Lawson Peters, Robert Cowan, Karina Needham

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Cochlear implant stimulation creates a reduction in electrode impedance that returns to pre-stimulation levels following cessation of stimulation and is presumed to be associated with the fibrous tissue covering over the electrode array. This study assessed the possibility that transitory impedance reduction originates from a change in the membrane permeability of cells on the electrode (electropermeabilization). These changes can be recorded using the dye propidium iodide, which fluoresces upon entry into the leaky cell. The in vitro model used showed impedance reduction and dye uptake into adherent cells overlying planar gold electrodes stimulated with as little as 5 min of clinically relevant cochlear implant stimulation. The delayed additions of propidium iodide showed a similar dye uptake to those groups with concurrent dye addition, suggesting the electropermeabilization was not reversible. Further understanding of the mechanisms behind these impedance and cell permeability changes with cochlear implant electrical stimulation may provide opportunities for creating longlasting reductions in electrode impedance.

Original languageEnglish
Pages (from-to)283-292
Number of pages10
JournalAudiology and Neurotology
Volume19
Issue number5
DOIs
Publication statusPublished - 2 Dec 2014
Externally publishedYes

Keywords

  • Adherent cells
  • Cochlear implant
  • Electrical stimulation
  • Electrode
  • Electropermeabilization

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