TY - JOUR
T1 - Electrospun nanodiamond-silk fibroin membranes
T2 - a multifunctional platform for biosensing and wound-healing applications
AU - Khalid, Asma
AU - Bai, Dongbi
AU - Abraham, Amanda N.
AU - Jadhav, Amit
AU - Linklater, Denver
AU - Matusica, Alex
AU - Nguyen, Duy
AU - Murdoch, Billy James
AU - Zakhartchouk, Nadia
AU - Dekiwadia, Chaitali
AU - Reineck, Philipp
AU - Simpson, David
AU - Vidanapathirana, Achini K.
AU - Houshyar, Shadi
AU - Bursill, Christina A.
AU - Ivanova, Elena P.
AU - Gibson, Brant C.
PY - 2020/10/28
Y1 - 2020/10/28
N2 - Next generation wound care technology capable of diagnosing wound parameters, promoting healthy cell growth, and reducing pathogenic infections noninvasively would provide patients with an improved standard of care and accelerated wound repair. Temperature is one of the indicating biomarkers specific to chronic wounds. This work reports a hybrid, multifunctional optical material platform - nanodiamond (ND)-silk membranes as biopolymer dressings capable of temperature sensing and promoting wound healing. The hybrid structure was fabricated through electrospinning, and 3D submicron fibrous membranes with high porosity were formed. Silk fibers are capable of compensating for the lack of an extracellular matrix at the wound site, supporting the wound-healing process. Negatively charged nitrogen vacancy (NV-) color centers in NDs exhibit optically detected magnetic resonance (ODMR) and act as nanoscale thermometers. This can be exploited to sense temperature variations associated with the presence of infection or inflammation in a wound, without physically removing the dressing. Our results show that the presence of NDs in the hybrid ND-silk membranes improves the thermal stability of silk fibers. NV- color centers in NDs embedded in silk fibers exhibit well-retained fluorescence and ODMR. Using the NV- centers as fluorescent nanoscale thermometers, we achieved temperature sensing in 25-50 °C, including the biologically relevant temperature window, for cell-grown ND-silk membranes. An enhancement (∼1.5× on average) in the temperature sensitivity of the NV- centers was observed for the hybrid materials. The hybrid membranes were further tested in vivo in a murine wound-healing model and demonstrated biocompatibility and equivalent wound closure rates as the control wounds. Additionally, the hybrid ND-silk membranes exhibited selective antifouling and biocidal propensity toward Gram-negative Pseudomonas aeruginosa and Escherichia coli, while no effect was observed on Gram-positive Staphylococcus aureus.
AB - Next generation wound care technology capable of diagnosing wound parameters, promoting healthy cell growth, and reducing pathogenic infections noninvasively would provide patients with an improved standard of care and accelerated wound repair. Temperature is one of the indicating biomarkers specific to chronic wounds. This work reports a hybrid, multifunctional optical material platform - nanodiamond (ND)-silk membranes as biopolymer dressings capable of temperature sensing and promoting wound healing. The hybrid structure was fabricated through electrospinning, and 3D submicron fibrous membranes with high porosity were formed. Silk fibers are capable of compensating for the lack of an extracellular matrix at the wound site, supporting the wound-healing process. Negatively charged nitrogen vacancy (NV-) color centers in NDs exhibit optically detected magnetic resonance (ODMR) and act as nanoscale thermometers. This can be exploited to sense temperature variations associated with the presence of infection or inflammation in a wound, without physically removing the dressing. Our results show that the presence of NDs in the hybrid ND-silk membranes improves the thermal stability of silk fibers. NV- color centers in NDs embedded in silk fibers exhibit well-retained fluorescence and ODMR. Using the NV- centers as fluorescent nanoscale thermometers, we achieved temperature sensing in 25-50 °C, including the biologically relevant temperature window, for cell-grown ND-silk membranes. An enhancement (∼1.5× on average) in the temperature sensitivity of the NV- centers was observed for the hybrid materials. The hybrid membranes were further tested in vivo in a murine wound-healing model and demonstrated biocompatibility and equivalent wound closure rates as the control wounds. Additionally, the hybrid ND-silk membranes exhibited selective antifouling and biocidal propensity toward Gram-negative Pseudomonas aeruginosa and Escherichia coli, while no effect was observed on Gram-positive Staphylococcus aureus.
KW - biocompatible materials
KW - electrospinning
KW - nanodiamonds
KW - nitrogen vacancy center
KW - silk fibroin
KW - temperature sensing
KW - wound healing
UR - http://www.scopus.com/inward/record.url?scp=85094631396&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/arc/LE140100131
U2 - 10.1021/acsami.0c15612
DO - 10.1021/acsami.0c15612
M3 - Article
C2 - 33047948
AN - SCOPUS:85094631396
SN - 1944-8244
VL - 12
SP - 48408
EP - 48419
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 43
ER -