'Empty' minicircles and petB/atpA and psbD/psbE (cytb559 α) genes in tandem in Amphidinium carterae plastid DNA

Roger G. Hiller*

*Corresponding author for this work

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Amphidinium carterae minicircle chloroplast DNA was separated from total DNA by centrifugation through a sucrose/NaCl gradient. Sequences of minicircles with psbA and 23S rRNA contained a common region of 67 bp. Primers designed from this generated numerous polymerase chain reaction products of 1.5-2.6 kb. These contained psaA and psaB as one gene/circle, and petB/atpA and psbD/psbE as two genes/circle. 'Empty' minicircles of 1.7-2.5 kb containing no identifiable genes or parts of genes were more abundant than gene-containing circles. From 15 minicircles a minimum common region of 48 bp was identified, with little identity to that from other dinoflagellate minicircles.

Original languageEnglish
Pages (from-to)449-452
Number of pages4
JournalFEBS Letters
Volume505
Issue number3
DOIs
Publication statusPublished - 21 Sep 2001

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