Abstract
A rapid and simple approach is presented to address the most critical issues of surface-enhanced Raman scattering (SERS)-based immunoassay such as removal/avoiding nonspecific adsorption and reducing assay time. The approach demonstrated involves rationally designed fluorophore integrated gold/silver nanoshells as SERS nanotags and utilizes alternative current electrohydrodynamic (ac-EHD) - induced nanoscaled surface shear forces to enhance the capture kinetics. The assay performance was validated in comparison with hydrodynamic flow and conventional immunoassay based devices. These nanoscaled physical forces acting within nanometer distances from the surface enabled rapid (40 min), sensitive (10 fg/mL) and highly specific detection of human epidermal growth factor receptor 2 (HER2) in breast cancer patient samples.
Original language | English |
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Title of host publication | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015) |
Place of Publication | San Diego, CA |
Publisher | Chemical and Biological Microsystems Society |
Pages | 257-259 |
Number of pages | 3 |
ISBN (Electronic) | 9780979806483 |
ISBN (Print) | 9781510817876 |
Publication status | Published - 2015 |
Externally published | Yes |
Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 |
Other
Other | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
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Country/Territory | Korea, Republic of |
City | Gyeongju |
Period | 25/10/15 → 29/10/15 |
Keywords
- Immunoassay
- Nonspecific adsorption
- SERS
- Shear force