Enabling rapid and specific SERS immunoassay using nano-scaled surface shear forces

Ramanathan Vaidyanathan, Yuling Wang*, Muhammad J. A. Shiddiky, Matt Trau

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

Abstract

A rapid and simple approach is presented to address the most critical issues of surface-enhanced Raman scattering (SERS)-based immunoassay such as removal/avoiding nonspecific adsorption and reducing assay time. The approach demonstrated involves rationally designed fluorophore integrated gold/silver nanoshells as SERS nanotags and utilizes alternative current electrohydrodynamic (ac-EHD) - induced nanoscaled surface shear forces to enhance the capture kinetics. The assay performance was validated in comparison with hydrodynamic flow and conventional immunoassay based devices. These nanoscaled physical forces acting within nanometer distances from the surface enabled rapid (40 min), sensitive (10 fg/mL) and highly specific detection of human epidermal growth factor receptor 2 (HER2) in breast cancer patient samples.

Original languageEnglish
Title of host publication19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
Place of PublicationSan Diego, CA
PublisherChemical and Biological Microsystems Society
Pages257-259
Number of pages3
ISBN (Electronic)9780979806483
ISBN (Print)9781510817876
Publication statusPublished - 2015
Externally publishedYes
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 25 Oct 201529 Oct 2015

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period25/10/1529/10/15

Keywords

  • Immunoassay
  • Nonspecific adsorption
  • SERS
  • Shear force

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