Enabling rapid and specific SERS immunoassay using nano-scaled surface shear forces

Ramanathan Vaidyanathan; Yuling Wang; Muhammad J. A. Shiddiky; Matt Trau

Enabling rapid and specific SERS immunoassay using nano-scaled surface shear forces

Ramanathan Vaidyanathan, Yuling Wang^*, Muhammad J. A. Shiddiky, Matt Trau

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution

Abstract

A rapid and simple approach is presented to address the most critical issues of surface-enhanced Raman scattering (SERS)-based immunoassay such as removal/avoiding nonspecific adsorption and reducing assay time. The approach demonstrated involves rationally designed fluorophore integrated gold/silver nanoshells as SERS nanotags and utilizes alternative current electrohydrodynamic (ac-EHD) - induced nanoscaled surface shear forces to enhance the capture kinetics. The assay performance was validated in comparison with hydrodynamic flow and conventional immunoassay based devices. These nanoscaled physical forces acting within nanometer distances from the surface enabled rapid (40 min), sensitive (10 fg/mL) and highly specific detection of human epidermal growth factor receptor 2 (HER2) in breast cancer patient samples.

Original language	English
Title of host publication	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
Place of Publication	San Diego, CA
Publisher	Chemical and Biological Microsystems Society
Pages	257-259
Number of pages	3
ISBN (Electronic)	9780979806483
ISBN (Print)	9781510817876
Publication status	Published - 2015
Externally published	Yes
Event	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015

Other

Other	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Country	Korea, Republic of
City	Gyeongju
Period	25/10/15 → 29/10/15

Keywords

Immunoassay
Nonspecific adsorption
SERS
Shear force

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title = "Enabling rapid and specific SERS immunoassay using nano-scaled surface shear forces",

abstract = "A rapid and simple approach is presented to address the most critical issues of surface-enhanced Raman scattering (SERS)-based immunoassay such as removal/avoiding nonspecific adsorption and reducing assay time. The approach demonstrated involves rationally designed fluorophore integrated gold/silver nanoshells as SERS nanotags and utilizes alternative current electrohydrodynamic (ac-EHD) - induced nanoscaled surface shear forces to enhance the capture kinetics. The assay performance was validated in comparison with hydrodynamic flow and conventional immunoassay based devices. These nanoscaled physical forces acting within nanometer distances from the surface enabled rapid (40 min), sensitive (10 fg/mL) and highly specific detection of human epidermal growth factor receptor 2 (HER2) in breast cancer patient samples.",

keywords = "Immunoassay, Nonspecific adsorption, SERS, Shear force",

author = "Ramanathan Vaidyanathan and Yuling Wang and Shiddiky, {Muhammad J. A.} and Matt Trau",

year = "2015",

language = "English",

isbn = "9781510817876",

pages = "257--259",

booktitle = "19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)",

publisher = "Chemical and Biological Microsystems Society",

}

Vaidyanathan, R, Wang, Y, Shiddiky, MJA & Trau, M 2015, Enabling rapid and specific SERS immunoassay using nano-scaled surface shear forces. in 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). Chemical and Biological Microsystems Society, San Diego, CA, pp. 257-259, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 25/10/15.

Enabling rapid and specific SERS immunoassay using nano-scaled surface shear forces. / Vaidyanathan, Ramanathan; Wang, Yuling; Shiddiky, Muhammad J. A.; Trau, Matt.

19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). San Diego, CA : Chemical and Biological Microsystems Society, 2015. p. 257-259.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution

TY - GEN

T1 - Enabling rapid and specific SERS immunoassay using nano-scaled surface shear forces

AU - Vaidyanathan, Ramanathan

AU - Wang, Yuling

AU - Shiddiky, Muhammad J. A.

AU - Trau, Matt

PY - 2015

Y1 - 2015

N2 - A rapid and simple approach is presented to address the most critical issues of surface-enhanced Raman scattering (SERS)-based immunoassay such as removal/avoiding nonspecific adsorption and reducing assay time. The approach demonstrated involves rationally designed fluorophore integrated gold/silver nanoshells as SERS nanotags and utilizes alternative current electrohydrodynamic (ac-EHD) - induced nanoscaled surface shear forces to enhance the capture kinetics. The assay performance was validated in comparison with hydrodynamic flow and conventional immunoassay based devices. These nanoscaled physical forces acting within nanometer distances from the surface enabled rapid (40 min), sensitive (10 fg/mL) and highly specific detection of human epidermal growth factor receptor 2 (HER2) in breast cancer patient samples.

AB - A rapid and simple approach is presented to address the most critical issues of surface-enhanced Raman scattering (SERS)-based immunoassay such as removal/avoiding nonspecific adsorption and reducing assay time. The approach demonstrated involves rationally designed fluorophore integrated gold/silver nanoshells as SERS nanotags and utilizes alternative current electrohydrodynamic (ac-EHD) - induced nanoscaled surface shear forces to enhance the capture kinetics. The assay performance was validated in comparison with hydrodynamic flow and conventional immunoassay based devices. These nanoscaled physical forces acting within nanometer distances from the surface enabled rapid (40 min), sensitive (10 fg/mL) and highly specific detection of human epidermal growth factor receptor 2 (HER2) in breast cancer patient samples.

KW - Immunoassay

KW - Nonspecific adsorption

KW - SERS

KW - Shear force

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M3 - Conference proceeding contribution

SN - 9781510817876

SP - 257

EP - 259

BT - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)

PB - Chemical and Biological Microsystems Society

CY - San Diego, CA

ER -