Enabling Rapid and Specific Surface-Enhanced Raman Scattering Immunoassay Using Nanoscaled Surface Shear Forces

Yuling Wang*, Ramanathan Vaidyanathan, Muhammad J A Shiddiky, Matt Trau

*Corresponding author for this work

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

A rapid and simple approach is presented to address two critical issues of surface-enhanced Raman scattering (SERS)-based immunoassay such as removal/avoiding nonspecific adsorption and reducing assay time. The approach demonstrated involves rationally designed fluorophore-integrated gold/silver nanoshells as SERS nanotags and utilizes alternative current electrohydrodynamic (ac-EHD)-induced nanoscaled surface shear forces to enhance the capture kinetics. The assay performance was validated in comparison with hydrodynamic flow and conventional immunoassay-based devices. These nanoscaled physical forces acting within nanometer distances from the electrode surface enabled rapid (40 min), sensitive (10 fg/mL), and highly specific detection of human epidermal growth factor receptor 2 in breast cancer patient samples. We believe this approach presents potential for the development of rapid and sensitive SERS immunoassays for routine clinical diagnosis.

Original languageEnglish
Pages (from-to)6354-6362
Number of pages9
JournalACS Nano
Volume9
Issue number6
DOIs
Publication statusPublished - 23 Jun 2015
Externally publishedYes

Keywords

  • ac-EHD
  • immunoassay
  • nonspecific adsorption
  • SERS
  • shear force

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