Photoreceptors are utilized by almost every organism to adapt to their ambient light environment. Our aim is to engineer a novel reversible molecular 'light switch' within E. coli by introducing a photoreceptor from non-photosynthetic bacteria (D. radiodurans and A. tumefaciens). By cloning the bacteriophytochrome coupled with heme-oxygenase, an enzyme that produces biliverdin from heme, the created colonies are able to respond to red and far-red light environments. This novel approach results in the colour of the E. coli 'switching' from blue to green. Our E. coli chameleon will serve as a fundamental 'bio-brick' for future applications by providing a simple and photo-reversible switch.
|Number of pages||1|
|Publication status||Published - 2010|
|Event||International Genetically Engineered Machine Competition - Cambridge, MA|
Duration: 6 Nov 2010 → 8 Nov 2010
|Conference||International Genetically Engineered Machine Competition|
|Period||6/11/10 → 8/11/10|
Aksoy, Y. A., Hare, J., Ibrahim, O., Mackenzie, K., Santhirasegaram, S., & Varinli, H. (2010). Engineering a bacteriophytochrome switch – creating a controllable E. coli chameleon. Poster session presented at International Genetically Engineered Machine Competition, Cambridge, MA, .