Enhanced colloidal stability and protein resistance of layered double hydroxide nanoparticles with phosphonic acid-terminated PEG coating for drug delivery

Zhenbang Cao, Nik Nik M. Adnan, Guoying Wang, Aditya Rawal, Bingyang Shi, Ruizhe Liu, Kang Liang, Lingyun Zhao, J. Justin Gooding, Cyrille Boyer, Zi Gu

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Conjugating nanoparticles with polyethylene glycol (PEG) is a useful strategy to improve the colloidal and biological stability of nanoparticles. However, studies on PEGylation of two-dimensional layered double hydroxide (LDH) nanoparticles are very limited. The present work reported two functionalization approaches to synthesize PEG-conjugated LDH nanoparticles by introducing phosphonic acid terminated PEG before and after LDH aging. The successful PEGylation was confirmed and suggested to be via electrostatic interaction and a ligand exchange process. Different functionalization approaches resulted in different binding types of PEG on/in LDH nanoparticles. The PEG coating maintained the dispersity of LDH nanoparticles in water and saline with the feeding mass ratio of 1:1. Further colloidal stability tests of PEGylated LDHs revealed that the PEGylated LDH dispersity was affected by the feeding mass ratio of PEG/LDH, the molar weight of PEG and anions intercalated in the LDHs. In a test to determine the extent of non-specific protein adsorption, the PEGylation was effective at resisting non-specific bovine serum albumin adsorption on LDH nanoparticles with both functionalization methods investigated. Moreover, PEGylated LDH nanoparticles had no effect on cell viability up to 500 µg/mL, and demonstrated enhanced cellular uptake in a SK-MEL-28 cell culture. The results in this work indicate that conjugating phosphonic acid-terminated PEG on LDH nanoparticles is a promising strategy to improve the colloidal and biological stability of LDHs for biomedical applications.

LanguageEnglish
Pages242-251
Number of pages10
JournalJournal of Colloid and Interface Science
Volume521
DOIs
Publication statusPublished - 1 Jul 2018

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Drug delivery
Polyethylene glycols
Nanoparticles
Proteins
Coatings
Acids
phosphonic acid
hydroxide ion
Adsorption
Coulomb interactions
Cell culture
Bovine Serum Albumin
Anions
Negative ions
Aging of materials
Ligands
Cells

Keywords

  • Layered double hydroxide
  • Nanoparticle
  • Non-specific protein adsorption
  • PEGylation
  • Stability

Cite this

Cao, Zhenbang ; Adnan, Nik Nik M. ; Wang, Guoying ; Rawal, Aditya ; Shi, Bingyang ; Liu, Ruizhe ; Liang, Kang ; Zhao, Lingyun ; Gooding, J. Justin ; Boyer, Cyrille ; Gu, Zi. / Enhanced colloidal stability and protein resistance of layered double hydroxide nanoparticles with phosphonic acid-terminated PEG coating for drug delivery. In: Journal of Colloid and Interface Science. 2018 ; Vol. 521. pp. 242-251.
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abstract = "Conjugating nanoparticles with polyethylene glycol (PEG) is a useful strategy to improve the colloidal and biological stability of nanoparticles. However, studies on PEGylation of two-dimensional layered double hydroxide (LDH) nanoparticles are very limited. The present work reported two functionalization approaches to synthesize PEG-conjugated LDH nanoparticles by introducing phosphonic acid terminated PEG before and after LDH aging. The successful PEGylation was confirmed and suggested to be via electrostatic interaction and a ligand exchange process. Different functionalization approaches resulted in different binding types of PEG on/in LDH nanoparticles. The PEG coating maintained the dispersity of LDH nanoparticles in water and saline with the feeding mass ratio of 1:1. Further colloidal stability tests of PEGylated LDHs revealed that the PEGylated LDH dispersity was affected by the feeding mass ratio of PEG/LDH, the molar weight of PEG and anions intercalated in the LDHs. In a test to determine the extent of non-specific protein adsorption, the PEGylation was effective at resisting non-specific bovine serum albumin adsorption on LDH nanoparticles with both functionalization methods investigated. Moreover, PEGylated LDH nanoparticles had no effect on cell viability up to 500 µg/mL, and demonstrated enhanced cellular uptake in a SK-MEL-28 cell culture. The results in this work indicate that conjugating phosphonic acid-terminated PEG on LDH nanoparticles is a promising strategy to improve the colloidal and biological stability of LDHs for biomedical applications.",
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Enhanced colloidal stability and protein resistance of layered double hydroxide nanoparticles with phosphonic acid-terminated PEG coating for drug delivery. / Cao, Zhenbang; Adnan, Nik Nik M.; Wang, Guoying; Rawal, Aditya; Shi, Bingyang; Liu, Ruizhe; Liang, Kang; Zhao, Lingyun; Gooding, J. Justin; Boyer, Cyrille; Gu, Zi.

In: Journal of Colloid and Interface Science, Vol. 521, 01.07.2018, p. 242-251.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Cao, Zhenbang

AU - Adnan, Nik Nik M.

AU - Wang, Guoying

AU - Rawal, Aditya

AU - Shi, Bingyang

AU - Liu, Ruizhe

AU - Liang, Kang

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AU - Gu, Zi

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