Enhanced fluorescent immunoassays on silver fractal-like structures

Tanya Shtoyko*, Evgenia G. Matveeva, I. Fen Chang, Zygmunt Gryczynski, Ewa Goldys, Ignacy Gryczynski

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)


Using the effect of the fluorescence enhancement in close proximity to metal nanostructures, we have been able to demonstrate ultrasensitive immunoassays suitable for the detection of biomarkers. Silver fractal-like structures have been grown by electrochemical reduction of silver on the surface of glass slides. A model immunoassay was performed on the slide surface with rabbit IgG (antigen) noncovalently immobilized on the slide, and rhodamine red-X-labeled antirabbit IgG conjugate was subsequently bound to the immobilized antigen. The fluorescence signal was measured from the glass-fractal's surface using a confocal microscope, and the images were compared to the images from the same surface not coated with fractals. Our results showed significant enhancement (more than 100-fold) of the signal detected on fractals compared to bare glass. We thus demonstrate that such fractal-like structures can assist in improving the signals from assays used in medical diagnostics, especially those for analytes with molecular weight under 100 kDa.

Original languageEnglish
Pages (from-to)1962-1966
Number of pages5
JournalAnalytical Chemistry
Issue number6
Publication statusPublished - 15 Mar 2008


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