Enhanced singlet oxygen generation from PLGA loaded with verteporfin and gold nanoparticles

Wei Deng, Zofia Kautzka, Ewa M. Goldys

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionResearchpeer-review

Abstract

In this study, poly(lactic-co-glycolic acid) (PLGA) nanocomposites were developed by incorporating a photosensitizer, verteporfin and gold nanoparticles into this polymeric matrix and utilised for enhanced photoynamic therapy. Both enhanced fluorescence and singlet oxygen generation from verteporfin were observed in this new formulation under both 425nm LED and 405nm laser illumination. A maximum enhancement factor of 2.5 for fluorescence and 1.84 for 1O2 generation was obtained when the molar ratio of gold:VP was 5:1 and excited at 425 nm, compared with PLGA doped with verteporfin only. The experiment results could be explained by the local electric field enhancement of gold nanoparticles. Furthermore, in vitro cell-killing effect on human pancreatic cancer cells was also demonstrated by using this new formulation following light exposure, indicating the utility of these nanocomposites for enhanced photodynamic therapy.

LanguageEnglish
Title of host publicationSPIE BioPhotonics Australasia
EditorsMark R. Hutchinson, Ewa M. Goldys
Place of PublicationS.l.
PublisherSPIE
Pages1-9
Number of pages9
ISBN (Electronic)9781510604346
DOIs
Publication statusPublished - 2016
EventSPIE BioPhotonics Australasia - Adelaide, Australia
Duration: 17 Oct 201619 Oct 2016

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume10013
ISSN (Print)0277-786X

Other

OtherSPIE BioPhotonics Australasia
CountryAustralia
CityAdelaide
Period17/10/1619/10/16

Fingerprint

Singlet Oxygen
Gold Nanoparticles
Nanocomposites
Gold
Fluorescence
Enhancement
Photodynamic Therapy
Photosensitizer
gold
Nanoparticles
nanoparticles
acids
Oxygen
Acids
Formulation
therapy
Cell
nanocomposites
oxygen
Local Field

Keywords

  • gold nanoparticles
  • metal nanostructure-enhanced fluorescence
  • photodynamic therapy
  • polymeric nanocomposites
  • singlet oxygen
  • verteporfin

Cite this

Deng, W., Kautzka, Z., & Goldys, E. M. (2016). Enhanced singlet oxygen generation from PLGA loaded with verteporfin and gold nanoparticles. In M. R. Hutchinson, & E. M. Goldys (Eds.), SPIE BioPhotonics Australasia (pp. 1-9). [100131O] (Proceedings of SPIE; Vol. 10013). S.l.: SPIE. https://doi.org/10.1117/12.2242362
Deng, Wei ; Kautzka, Zofia ; Goldys, Ewa M. / Enhanced singlet oxygen generation from PLGA loaded with verteporfin and gold nanoparticles. SPIE BioPhotonics Australasia. editor / Mark R. Hutchinson ; Ewa M. Goldys. S.l. : SPIE, 2016. pp. 1-9 (Proceedings of SPIE).
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abstract = "In this study, poly(lactic-co-glycolic acid) (PLGA) nanocomposites were developed by incorporating a photosensitizer, verteporfin and gold nanoparticles into this polymeric matrix and utilised for enhanced photoynamic therapy. Both enhanced fluorescence and singlet oxygen generation from verteporfin were observed in this new formulation under both 425nm LED and 405nm laser illumination. A maximum enhancement factor of 2.5 for fluorescence and 1.84 for 1O2 generation was obtained when the molar ratio of gold:VP was 5:1 and excited at 425 nm, compared with PLGA doped with verteporfin only. The experiment results could be explained by the local electric field enhancement of gold nanoparticles. Furthermore, in vitro cell-killing effect on human pancreatic cancer cells was also demonstrated by using this new formulation following light exposure, indicating the utility of these nanocomposites for enhanced photodynamic therapy.",
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Deng, W, Kautzka, Z & Goldys, EM 2016, Enhanced singlet oxygen generation from PLGA loaded with verteporfin and gold nanoparticles. in MR Hutchinson & EM Goldys (eds), SPIE BioPhotonics Australasia., 100131O, Proceedings of SPIE, vol. 10013, SPIE, S.l., pp. 1-9, SPIE BioPhotonics Australasia, Adelaide, Australia, 17/10/16. https://doi.org/10.1117/12.2242362

Enhanced singlet oxygen generation from PLGA loaded with verteporfin and gold nanoparticles. / Deng, Wei; Kautzka, Zofia; Goldys, Ewa M.

SPIE BioPhotonics Australasia. ed. / Mark R. Hutchinson; Ewa M. Goldys. S.l. : SPIE, 2016. p. 1-9 100131O (Proceedings of SPIE; Vol. 10013).

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionResearchpeer-review

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Deng W, Kautzka Z, Goldys EM. Enhanced singlet oxygen generation from PLGA loaded with verteporfin and gold nanoparticles. In Hutchinson MR, Goldys EM, editors, SPIE BioPhotonics Australasia. S.l.: SPIE. 2016. p. 1-9. 100131O. (Proceedings of SPIE). https://doi.org/10.1117/12.2242362