Enzyme production by recombinant Trichoderma reesei strains

Jaana M. Uusitalo*, K. M. Helena Nevalainen, Anu M. Harkki, Jonathan K C Knowles, Merja E. Penttilä

*Corresponding author for this work

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.

Original languageEnglish
Pages (from-to)35-49
Number of pages15
JournalJournal of Biotechnology
Volume17
Issue number1
DOIs
Publication statusPublished - 1991
Externally publishedYes

Keywords

  • Cellulase
  • Chymosin
  • Filamentous fungi
  • Heterologous gene expression
  • Trichoderma reesei

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