Equilibrium between cell division and apoptosis in immortal cells as an alternative to the G1 restriction mechanism in mammalian cells

Vadim N. Dedov*, Irina V. Dedova, Garth A. Nicholson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Starvation arrests cultured mammalian cells in the G1 restriction point of the cell cycle, whereas cancer cells generally lose the regulatory control of the cell cycle. Human lymphocytes, infecfed with Epstein-Barr virus (EBV), also lose their cell cycle control and produce immortal lymphoblastoid cell lines. We show that during starvation, EBV-lymphoblasts override the cell cycle arrest in the G1 restriction point and continue cell division. Simultaneously, starvation activates apoptosis in an approximately half of the daughter cells in each cell generation. Continuos cell division and partial removal of cells by apoptosis results in stabilization of viable cell numbers, where a majority of viable cells are in the G1 phase of the cell cycle. In contrast to starvation, anticancer drug etoposide activates apoptosis indiscriminately in all EBV-lymphoblasts and convertes all the viable cells into apoptotic. We conclude that the removal of surplus cells by apoptosis may represent a survival mechanism of transformed (i.e., cancer) cell population in nutrient restricted conditions, whereas non-transformed mammalian cells are arrested in the G 1 restriction point of the cell cycle.

Original languageEnglish
Pages (from-to)491-495
Number of pages5
JournalCell Cycle
Volume3
Issue number4
DOIs
Publication statusPublished - 2004
Externally publishedYes

Keywords

  • Apoptosis
  • Cell cycle
  • EBV-lymphoblasts
  • Epstein-Barr virus
  • Restriction point
  • Starvation

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