A multilayered synthetic human elastin/polycaprolactone hybrid vascular graft with tailored mechanical properties

Steven G. Wise, Michael J. Byrom, Anna Waterhouse, Paul G. Bannon, Martin K. C. Ng, Anthony S. Weiss

Research output: Contribution to journalArticlepeer-review

202 Citations (Scopus)

Abstract

Small-diameter synthetic vascular graft materials fail to match the patency of human tissue conduits used in vascular bypass surgery. The foreign surface retards endothelialization and is highly thrombogenic, while the mismatch in mechanical properties induces intimal hyperplasia. Using recombinant human tropoelastin, we have developed a synthetic vascular conduit for small-diameter applications. We show that tropoelastin enhances endothelial cell attachment (threefold vs. control) and proliferation by 54.7 ± 1.1% (3 days vs. control). Tropoelastin, when presented as a monomer and when cross-linked into synthetic elastin for biomaterials applications, had low thrombogenicity. Activation of the intrinsic pathway of coagulation, measured by plasma clotting time, was reduced for tropoelastin (60.4 ± 8.2% vs. control). Platelet attachment was also reduced compared to collagen. Reductions in platelet interactions were mirrored on cross-linked synthetic elastin scaffolds. Tropoelastin was subsequently incorporated into a synthetic elastin/ polycaprolactone conduit with mechanical properties optimized to mimic the human internal mammary artery, including permeability, compliance, elastic modulus and burst pressure. Further, this multilayered conduit presented a synthetic elastin internal lamina to circulating blood and demonstrated suturability and mechanical durability in a small scale rabbit carotid interposition model.

Original languageEnglish
Pages (from-to)295-303
Number of pages9
JournalActa Biomaterialia
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 2011
Externally publishedYes

Bibliographical note

Erratum can be found in Acta Biomaterialia, Volume 7(3), 1429,
http://dx.doi.org/10.1016/j.actbio.2010.11.009

Keywords

  • artery
  • blood vessel
  • conduit
  • elastin
  • graft

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