Establishment of a taxonomic and molecular reference collection to support the identification of species regulated by the Western Australian Prevention List for Introduced Marine Pests

P. Joana Dias, Seema Fotedar, Julieta Munoz, Matthew J. Hewitt, Sherralee Lukehurst, Mathew Hourston, Claire Wellington, Roger Duggan, Samantha Bridgwood, Marion Massam, Victoria Aitken, Paul de Lestang, Simon McKirdy, Richard Willan, Lisa Kirkendale, Jennifer Giannetta, Maria Corsini-Foka, Steve Pothoven, Fiona Gower, Frédérique Viard & 23 others Christian Buschbaum, Giuseppe Scarcella, Pierluigi Strafella, Melanie J. Bishop, Timothy Sullivan, Isabella Buttino, Hawis Madduppa, Mareike Huhn, Chela J. Zabin, Karolina Bacela-Spychalska, Dagmara Wójcik-Fudalewska, Alexandra Markert, Alexey Maximov, Lena Kautsky, Cornelia Jaspers, Jonne Kotta, Merli Pärnoja, Daniel Robledo, Konstantinos Tsiamis, Frithjof C. Küpper, Ante Žuljević, Justin I. McDonald, Michael Snow

Research output: Contribution to journalConference paperResearchpeer-review

Abstract

Introduced Marine Pests (IMP, = non-indigenous marine species) prevention, early detection and risk-based management strategies have become the priority for biosecurity operations worldwide, in recognition of the fact that, once established, the effective management of marine pests can rapidly become cost prohibitive or impractical. In Western Australia (WA), biosecurity management is guided by the “Western Australian Prevention List for Introduced Marine Pests” which is a policy tool that details species or genera as being of high risk to the region. This list forms the basis of management efforts to prevent introduction of these species, monitoring efforts to detect them at an early stage, and rapid response should they be detected. It is therefore essential that the species listed can be rapid and confidently identified and discriminated from native species by a range of government and industry stakeholders. Recognising that identification of these species requires very specialist expertise which may be in short supply and not readily accessible in a regulatory environment, and the fact that much publicly available data is not verifiable or suitable for regulatory enforcement, the WA government commissioned the current project to collate a reference collection of these marine pest specimens. In this work, we thus established collaboration with researchers worldwide in order to source representative specimens of the species listed. Our main objective was to build a reference collection of taxonomically vouchered specimens and subsequently to generate species-specific DNA barcodes suited to supporting their future identification. To date, we were able to obtain specimens of 75 species (representative of all but four of the pests listed) which have been identified by experts and placed with the WA Government Department of Fisheries and, where possible, in accessible museums and institutions in Australasia. The reference collection supports the fast and reliable taxonomic and molecular identification of marine pests in WA and constitutes a valuable resource for training of stakeholders with interest in IMP recognition in Australia. The reference collection is also useful in supporting the development of a variety of DNA-based detection strategies such as real-time PCR and metabarcoding of complex environmental samples (e.g. biofouling communities). The Prevention List is under regular review to ensure its continued relevance and that it remains evidence and risk-based. Similarly, its associated reference collection also remains to some extent a work in progress. In recognition of this fact, this report seeks to provide details of this continually evolving information repository publicly available to the biosecurity management community worldwide.

LanguageEnglish
Pages215-225
Number of pages11
JournalManagement of Biological Invasions
Volume8
Issue number2
DOIs
Publication statusPublished - Jun 2017
EventInternational Conference on Marine Bioinvasions (9th : 2017) - Sydney, Australia
Duration: 19 Jan 201621 Jan 2016

Fingerprint

pests
Western Australia
biosecurity
stakeholders
biofouling
stakeholder
Australasian region
DNA barcoding
DNA
species identification
pest
quantitative polymerase chain reaction
indigenous species
researchers
fisheries
repository
native species
museum
industry
monitoring

Bibliographical note

Copyright the Author(s) 2017. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • Introduced Marine Pests (IMP)
  • taxonomic voucher
  • cytochrome c oxidase I
  • COI
  • The Barcode of Life Data System
  • BoLD
  • marine biosecurity

Cite this

Dias, P. Joana ; Fotedar, Seema ; Munoz, Julieta ; Hewitt, Matthew J. ; Lukehurst, Sherralee ; Hourston, Mathew ; Wellington, Claire ; Duggan, Roger ; Bridgwood, Samantha ; Massam, Marion ; Aitken, Victoria ; de Lestang, Paul ; McKirdy, Simon ; Willan, Richard ; Kirkendale, Lisa ; Giannetta, Jennifer ; Corsini-Foka, Maria ; Pothoven, Steve ; Gower, Fiona ; Viard, Frédérique ; Buschbaum, Christian ; Scarcella, Giuseppe ; Strafella, Pierluigi ; Bishop, Melanie J. ; Sullivan, Timothy ; Buttino, Isabella ; Madduppa, Hawis ; Huhn, Mareike ; Zabin, Chela J. ; Bacela-Spychalska, Karolina ; Wójcik-Fudalewska, Dagmara ; Markert, Alexandra ; Maximov, Alexey ; Kautsky, Lena ; Jaspers, Cornelia ; Kotta, Jonne ; Pärnoja, Merli ; Robledo, Daniel ; Tsiamis, Konstantinos ; Küpper, Frithjof C. ; Žuljević, Ante ; McDonald, Justin I. ; Snow, Michael. / Establishment of a taxonomic and molecular reference collection to support the identification of species regulated by the Western Australian Prevention List for Introduced Marine Pests. In: Management of Biological Invasions. 2017 ; Vol. 8, No. 2. pp. 215-225.
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abstract = "Introduced Marine Pests (IMP, = non-indigenous marine species) prevention, early detection and risk-based management strategies have become the priority for biosecurity operations worldwide, in recognition of the fact that, once established, the effective management of marine pests can rapidly become cost prohibitive or impractical. In Western Australia (WA), biosecurity management is guided by the “Western Australian Prevention List for Introduced Marine Pests” which is a policy tool that details species or genera as being of high risk to the region. This list forms the basis of management efforts to prevent introduction of these species, monitoring efforts to detect them at an early stage, and rapid response should they be detected. It is therefore essential that the species listed can be rapid and confidently identified and discriminated from native species by a range of government and industry stakeholders. Recognising that identification of these species requires very specialist expertise which may be in short supply and not readily accessible in a regulatory environment, and the fact that much publicly available data is not verifiable or suitable for regulatory enforcement, the WA government commissioned the current project to collate a reference collection of these marine pest specimens. In this work, we thus established collaboration with researchers worldwide in order to source representative specimens of the species listed. Our main objective was to build a reference collection of taxonomically vouchered specimens and subsequently to generate species-specific DNA barcodes suited to supporting their future identification. To date, we were able to obtain specimens of 75 species (representative of all but four of the pests listed) which have been identified by experts and placed with the WA Government Department of Fisheries and, where possible, in accessible museums and institutions in Australasia. The reference collection supports the fast and reliable taxonomic and molecular identification of marine pests in WA and constitutes a valuable resource for training of stakeholders with interest in IMP recognition in Australia. The reference collection is also useful in supporting the development of a variety of DNA-based detection strategies such as real-time PCR and metabarcoding of complex environmental samples (e.g. biofouling communities). The Prevention List is under regular review to ensure its continued relevance and that it remains evidence and risk-based. Similarly, its associated reference collection also remains to some extent a work in progress. In recognition of this fact, this report seeks to provide details of this continually evolving information repository publicly available to the biosecurity management community worldwide.",
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Dias, PJ, Fotedar, S, Munoz, J, Hewitt, MJ, Lukehurst, S, Hourston, M, Wellington, C, Duggan, R, Bridgwood, S, Massam, M, Aitken, V, de Lestang, P, McKirdy, S, Willan, R, Kirkendale, L, Giannetta, J, Corsini-Foka, M, Pothoven, S, Gower, F, Viard, F, Buschbaum, C, Scarcella, G, Strafella, P, Bishop, MJ, Sullivan, T, Buttino, I, Madduppa, H, Huhn, M, Zabin, CJ, Bacela-Spychalska, K, Wójcik-Fudalewska, D, Markert, A, Maximov, A, Kautsky, L, Jaspers, C, Kotta, J, Pärnoja, M, Robledo, D, Tsiamis, K, Küpper, FC, Žuljević, A, McDonald, JI & Snow, M 2017, 'Establishment of a taxonomic and molecular reference collection to support the identification of species regulated by the Western Australian Prevention List for Introduced Marine Pests' Management of Biological Invasions, vol. 8, no. 2, pp. 215-225. https://doi.org/10.3391/mbi.2017.8.2.09

Establishment of a taxonomic and molecular reference collection to support the identification of species regulated by the Western Australian Prevention List for Introduced Marine Pests. / Dias, P. Joana; Fotedar, Seema; Munoz, Julieta; Hewitt, Matthew J.; Lukehurst, Sherralee; Hourston, Mathew; Wellington, Claire; Duggan, Roger; Bridgwood, Samantha; Massam, Marion; Aitken, Victoria; de Lestang, Paul; McKirdy, Simon; Willan, Richard; Kirkendale, Lisa; Giannetta, Jennifer; Corsini-Foka, Maria; Pothoven, Steve; Gower, Fiona; Viard, Frédérique; Buschbaum, Christian; Scarcella, Giuseppe; Strafella, Pierluigi; Bishop, Melanie J.; Sullivan, Timothy; Buttino, Isabella; Madduppa, Hawis; Huhn, Mareike; Zabin, Chela J.; Bacela-Spychalska, Karolina; Wójcik-Fudalewska, Dagmara; Markert, Alexandra; Maximov, Alexey; Kautsky, Lena; Jaspers, Cornelia; Kotta, Jonne; Pärnoja, Merli; Robledo, Daniel; Tsiamis, Konstantinos; Küpper, Frithjof C.; Žuljević, Ante; McDonald, Justin I.; Snow, Michael.

In: Management of Biological Invasions, Vol. 8, No. 2, 06.2017, p. 215-225.

Research output: Contribution to journalConference paperResearchpeer-review

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AU - Fotedar,Seema

AU - Munoz,Julieta

AU - Hewitt,Matthew J.

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AU - Markert,Alexandra

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