TY - JOUR
T1 - Evaluating irradiation dose for sterility induction and quality control of mass-produced fruit fly bactrocera tryoni (Diptera:Tephritidae)
AU - Dominiak, B. C.
AU - Sundaralingam, S.
AU - Jiang, L.
AU - Fanson, B. G.
AU - Collins, S. R.
AU - Banos, C.
AU - Davies, J. B.
AU - Taylor, P. W.
PY - 2014/6/1
Y1 - 2014/6/1
N2 - The sterile insect technique has been routinely used to eradicate fruit fly Bactrocera tryoni (Froggatt) incursions. This study considers whether fly quality in a mass-rearing facility can be improved by reducing irradiation doses, without sacrificing reproductive sterility. Pupae were exposed to one of five target irradiation dose ranges: 0, 40-45, 50-55, 60-65, and 70-75 Gy. Pupae were then assessed using routine quality control measures: flight ability, sex ratio, longevity under nutritional stress, emergence, and reproductive sterility. Irradiation did not have a significant effect on flight ability or sex ratio tests. Longevity under nutritional stress was significantly increased at 70-75 Gy, but no other doses differed from 0 Gy. Emergence was slightly reduced in the 50-55,60-65, and 70 -75 Gy treatments, but 40-45 Gy treatments did not differ from 0 Gy, though confounding temporal factors complicate interpretation. Reproductive sterility remained acceptable (>99.5%) for all doses-40-45 Gy (99.78%), 50-55 Gy (100%), 60-65 Gy (100%), and 70-75 Gy (99.99%). We recommend that B. tryoni used in sterile insect technique releases be irradiated at a target dose of 50-55 Gy, providing improved quality and undiminished sterility in comparison with the current 70-75 Gy standard while also providing a substantial buffer against risk of under dosing.
AB - The sterile insect technique has been routinely used to eradicate fruit fly Bactrocera tryoni (Froggatt) incursions. This study considers whether fly quality in a mass-rearing facility can be improved by reducing irradiation doses, without sacrificing reproductive sterility. Pupae were exposed to one of five target irradiation dose ranges: 0, 40-45, 50-55, 60-65, and 70-75 Gy. Pupae were then assessed using routine quality control measures: flight ability, sex ratio, longevity under nutritional stress, emergence, and reproductive sterility. Irradiation did not have a significant effect on flight ability or sex ratio tests. Longevity under nutritional stress was significantly increased at 70-75 Gy, but no other doses differed from 0 Gy. Emergence was slightly reduced in the 50-55,60-65, and 70 -75 Gy treatments, but 40-45 Gy treatments did not differ from 0 Gy, though confounding temporal factors complicate interpretation. Reproductive sterility remained acceptable (>99.5%) for all doses-40-45 Gy (99.78%), 50-55 Gy (100%), 60-65 Gy (100%), and 70-75 Gy (99.99%). We recommend that B. tryoni used in sterile insect technique releases be irradiated at a target dose of 50-55 Gy, providing improved quality and undiminished sterility in comparison with the current 70-75 Gy standard while also providing a substantial buffer against risk of under dosing.
KW - Bactrocera
KW - Tephritidae
KW - insect quality parameter
KW - mass production
KW - sterile insect technique
UR - http://www.scopus.com/inward/record.url?scp=84901746662&partnerID=8YFLogxK
U2 - 10.1603/EC13421
DO - 10.1603/EC13421
M3 - Article
VL - 107
SP - 1172
EP - 1178
JO - Journal of economic entomology
JF - Journal of economic entomology
SN - 0022-0493
IS - 3
ER -