Evaluating irradiation dose for sterility induction and quality control of mass-produced fruit fly bactrocera tryoni (Diptera:Tephritidae)

B. C. Dominiak*, S. Sundaralingam, L. Jiang, B. G. Fanson, S. R. Collins, C. Banos, J. B. Davies, P. W. Taylor

*Corresponding author for this work

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The sterile insect technique has been routinely used to eradicate fruit fly Bactrocera tryoni (Froggatt) incursions. This study considers whether fly quality in a mass-rearing facility can be improved by reducing irradiation doses, without sacrificing reproductive sterility. Pupae were exposed to one of five target irradiation dose ranges: 0, 40-45, 50-55, 60-65, and 70-75 Gy. Pupae were then assessed using routine quality control measures: flight ability, sex ratio, longevity under nutritional stress, emergence, and reproductive sterility. Irradiation did not have a significant effect on flight ability or sex ratio tests. Longevity under nutritional stress was significantly increased at 70-75 Gy, but no other doses differed from 0 Gy. Emergence was slightly reduced in the 50-55,60-65, and 70 -75 Gy treatments, but 40-45 Gy treatments did not differ from 0 Gy, though confounding temporal factors complicate interpretation. Reproductive sterility remained acceptable (>99.5%) for all doses-40-45 Gy (99.78%), 50-55 Gy (100%), 60-65 Gy (100%), and 70-75 Gy (99.99%). We recommend that B. tryoni used in sterile insect technique releases be irradiated at a target dose of 50-55 Gy, providing improved quality and undiminished sterility in comparison with the current 70-75 Gy standard while also providing a substantial buffer against risk of under dosing.

Original languageEnglish
Pages (from-to)1172-1178
Number of pages7
JournalJournal of economic entomology
Volume107
Issue number3
DOIs
Publication statusPublished - 1 Jun 2014

Keywords

  • Bactrocera
  • Tephritidae
  • insect quality parameter
  • mass production
  • sterile insect technique

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