@inbook{8aa4ce6995734485a1f899e53efb7f73,
title = "Evaluating opioid-mediated adenylyl cyclase inhibition in live cells using a BRET-based assay",
abstract = "Quantitative measurement of receptor signaling by different ligands is important for understanding the mechanism of drug action and screening of drugs. Here, we describe a simple and cost-effective method of measuring adenylyl cyclase inhibition, one of the hallmarks of opioid receptor activation. The assay is based on bioluminescence resonance energy transfer (BRET) that involves transfection of a biosensor in human embryonic kidney (HEK)-293 cells stably transfected with μ-opioid receptor (μ receptor), enabling real-time measurement of cAMP levels.",
keywords = "Adenylyl cyclase, BRET assay, cAMP, Forskolin, HEK-293 cells, μ-Opioid receptor",
author = "Preeti Manandhar and Shivani Sachdev and Marina Santiago",
year = "2021",
doi = "10.1007/978-1-0716-0884-5_11",
language = "English",
isbn = "9781071608838",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "117--125",
editor = "Spampinato, {Santi M.}",
booktitle = "Opioid receptors",
edition = "2nd",
}