TY - JOUR
T1 - Evaluation of a carbon nanotube-titanate nanotube nanocomposite as an electrochemical biosensor scaffold
AU - Liu, Xiaoqiang
AU - Yan, Rui
AU - Zhang, Jiamei
AU - Zhu, Jie
AU - Wong, Danny K Y
PY - 2015/4/15
Y1 - 2015/4/15
N2 - A significant aspect of this work is the development of a multi-wall carbon nanotube (MWCNT)-titanate nanotube (TNT) nanocomposite to serve as a biocompatible scaffold with high conductivity on a biosensor surface. Unlike other scaffolds consisting of MWCNTs alone or TNTs alone, the MWCNT-TNT nanocomposite synergistically provides excellent biocompatibility, good electrical conductivity, low electrochemical interferences and a high signal-to-noise ratio. For comparison, after characterising a scaffold consisting of MWCNTs alone, TNTs alone and a MWCNT-TNT nanocomposite using several spectroscopic techniques, the analytical performance of a horseradish peroxidase (HRP) electrochemical biosensor was evaluated using cyclic voltammetry and differential pulse voltammetry. The scaffold consisting of MWCNTs alone displayed a high background charging current, a low signal-to-noise ratio and distinct electrochemical interference from its surface functional groups. In contrast, the direct electrochemistry and the catalytic capability of HRP at MWCNT-TNT modified biosensors towards H2O2 was demonstrated to be ~51% and ~144% enhanced, respectively, compared to those at TNT modified biosensors. Meanwhile, MWCNT-TNT nanocomposite modified HRP biosensors also exhibited higher sensitivity (4.42μAmM-1) than TNT modified HRP biosensors (1.48μAmM-1). The above superior performance was attributed to the improved properties of MWCNT-TNT nanocomposite as biosensor scaffold compared to its two individual components by complementing each component and synergistically sustaining the characteristic features of each component.
AB - A significant aspect of this work is the development of a multi-wall carbon nanotube (MWCNT)-titanate nanotube (TNT) nanocomposite to serve as a biocompatible scaffold with high conductivity on a biosensor surface. Unlike other scaffolds consisting of MWCNTs alone or TNTs alone, the MWCNT-TNT nanocomposite synergistically provides excellent biocompatibility, good electrical conductivity, low electrochemical interferences and a high signal-to-noise ratio. For comparison, after characterising a scaffold consisting of MWCNTs alone, TNTs alone and a MWCNT-TNT nanocomposite using several spectroscopic techniques, the analytical performance of a horseradish peroxidase (HRP) electrochemical biosensor was evaluated using cyclic voltammetry and differential pulse voltammetry. The scaffold consisting of MWCNTs alone displayed a high background charging current, a low signal-to-noise ratio and distinct electrochemical interference from its surface functional groups. In contrast, the direct electrochemistry and the catalytic capability of HRP at MWCNT-TNT modified biosensors towards H2O2 was demonstrated to be ~51% and ~144% enhanced, respectively, compared to those at TNT modified biosensors. Meanwhile, MWCNT-TNT nanocomposite modified HRP biosensors also exhibited higher sensitivity (4.42μAmM-1) than TNT modified HRP biosensors (1.48μAmM-1). The above superior performance was attributed to the improved properties of MWCNT-TNT nanocomposite as biosensor scaffold compared to its two individual components by complementing each component and synergistically sustaining the characteristic features of each component.
KW - Catalytic capability
KW - Direct electrochemistry
KW - Electrochemical biosensor
KW - Immobilisation materials
KW - MWCNT-TNT nanocomposites
UR - http://www.scopus.com/inward/record.url?scp=84911879074&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2014.11.028
DO - 10.1016/j.bios.2014.11.028
M3 - Article
C2 - 25460903
AN - SCOPUS:84911879074
SN - 0956-5663
VL - 66
SP - 208
EP - 215
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -