Evaluation of a PDMS-based sensor for human CD4+-cell count

Sara Thorslund, Rolf Larsson, Fredrik Nikolajeff, Jonas Bergquist, Javier Sanchez

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

Abstract

Up to today, the number of CD4+ lymphocytes remains the most important biological marker to determine the clinical stage of an HIV-infection. Analysis by flow cytometry, the standard method used today, is unsuitable in many developing countries, because of high costs involved and practical inconveniences. We here describe a method where surface modified PDMS captures exclusively CD4+ cells from non-diluted human whole blood. A simple fluorescence microscope for stained leucocytes counting is the only detection equipment needed.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherJapan Academic Association Inc
Pages326-328
Number of pages3
Publication statusPublished - 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 5 Nov 20069 Nov 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period5/11/069/11/06

Keywords

  • CD4 cells
  • Heparin
  • HIV
  • PDMS
  • Point-of-care

Fingerprint Dive into the research topics of 'Evaluation of a PDMS-based sensor for human CD4+-cell count'. Together they form a unique fingerprint.

Cite this