Evaluation of a PDMS-based sensor for human CD4+-cell count

Sara Thorslund; Rolf Larsson; Fredrik Nikolajeff; Jonas Bergquist; Javier Sanchez

Evaluation of a PDMS-based sensor for human CD4+-cell count

Sara Thorslund, Rolf Larsson, Fredrik Nikolajeff, Jonas Bergquist, Javier Sanchez

Department of Physics and Astronomy

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution

Abstract

Up to today, the number of CD4⁺ lymphocytes remains the most important biological marker to determine the clinical stage of an HIV-infection. Analysis by flow cytometry, the standard method used today, is unsuitable in many developing countries, because of high costs involved and practical inconveniences. We here describe a method where surface modified PDMS captures exclusively CD4⁺ cells from non-diluted human whole blood. A simple fluorescence microscope for stained leucocytes counting is the only detection equipment needed.

Original language	English
Title of host publication	Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Japan Academic Association Inc
Pages	326-328
Number of pages	3
Publication status	Published - 2006
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 5 Nov 2006 → 9 Nov 2006

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country	Japan
City	Tokyo
Period	5/11/06 → 9/11/06

Keywords

CD4 cells
Heparin
HIV
PDMS
Point-of-care

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title = "Evaluation of a PDMS-based sensor for human CD4+-cell count",

abstract = "Up to today, the number of CD4+ lymphocytes remains the most important biological marker to determine the clinical stage of an HIV-infection. Analysis by flow cytometry, the standard method used today, is unsuitable in many developing countries, because of high costs involved and practical inconveniences. We here describe a method where surface modified PDMS captures exclusively CD4+ cells from non-diluted human whole blood. A simple fluorescence microscope for stained leucocytes counting is the only detection equipment needed.",

keywords = "CD4 cells, Heparin, HIV, PDMS, Point-of-care",

author = "Sara Thorslund and Rolf Larsson and Fredrik Nikolajeff and Jonas Bergquist and Javier Sanchez",

year = "2006",

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pages = "326--328",

booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

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Thorslund, S, Larsson, R, Nikolajeff, F, Bergquist, J & Sanchez, J 2006, Evaluation of a PDMS-based sensor for human CD4+-cell count. in Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, pp. 326-328, 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 5/11/06.

Evaluation of a PDMS-based sensor for human CD4+-cell count. / Thorslund, Sara; Larsson, Rolf; Nikolajeff, Fredrik; Bergquist, Jonas; Sanchez, Javier.

Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. p. 326-328.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution

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T1 - Evaluation of a PDMS-based sensor for human CD4+-cell count

AU - Thorslund, Sara

AU - Larsson, Rolf

AU - Nikolajeff, Fredrik

AU - Bergquist, Jonas

AU - Sanchez, Javier

PY - 2006

Y1 - 2006

N2 - Up to today, the number of CD4+ lymphocytes remains the most important biological marker to determine the clinical stage of an HIV-infection. Analysis by flow cytometry, the standard method used today, is unsuitable in many developing countries, because of high costs involved and practical inconveniences. We here describe a method where surface modified PDMS captures exclusively CD4+ cells from non-diluted human whole blood. A simple fluorescence microscope for stained leucocytes counting is the only detection equipment needed.

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KW - CD4 cells

KW - Heparin

KW - HIV

KW - PDMS

KW - Point-of-care

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M3 - Conference proceeding contribution

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BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Japan Academic Association Inc

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