Evaluation of resistance of oilseed rape genotypes to turnip yellows virus

Emad Ibrahim; Andrea Rychlá; Glenda Alquicer; Lucie Slavíková; Qi Peng; Miroslav Klíma; Viktor Vrbovský; Piotr Trebicki; Jiban Kumar Kundu

doi:10.3390/plants12132501

Evaluation of resistance of oilseed rape genotypes to turnip yellows virus

Emad Ibrahim, Andrea Rychlá, Glenda Alquicer, Lucie Slavíková, Qi Peng, Miroslav Klíma, Viktor Vrbovský, Piotr Trebicki, Jiban Kumar Kundu^*

^*Corresponding author for this work

Applied BioSciences

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Abstract

Turnip yellows virus (TuYV), is one of the most important pathogens of oilseed rape, which has caused enormous yield losses in all growing regions of the world in recent years. Therefore, there is a need for resistant varieties for sustainable crop protection. We have investigated the resistance of known varieties and newly developed advanced-breeding lines of oilseed rape to TuYV in greenhouse and field trials. We have analysed the TuYV titre of individual genotypes inoculated with the virus using viruliferous aphids Myzus persicae. The genotypes ‘DK Temptation’ and ‘Rescator’ had the lowest and highest virus titres, respectively, and were used as resistant and susceptible models for comparative analyses with other genotypes. In the greenhouse, the best results were obtained with the genotypes ‘OP-8143 DH’ (2.94 × 10⁵ copies), OP-BN-72 (3.29 × 10⁵ copies), ‘Navajo’ (3.58 × 10⁵ copies) and ‘SG-C 21215’ (4.09 × 10⁵ copies), which reached virus titres about 2 times higher than the minimum virus concentration measured in ‘DK Temptation’ (1.80 × 10⁵ copies). In the field trials, the genotypes ‘Navajo’ (3.39 × 10⁵ copies), ‘OP-8148 DH’ (4.44 × 10⁵ copies), ‘SG-C 21215’ (6.80 × 10⁵ copies) and OP-8480 (7.19 × 10⁵ copies) had the lowest virus titres and reached about 3 times the virus titre of DK Temptation (2.54 × 10⁵ copies). Both trials showed that at least two commercial varieties (e.g., DK Temptation, Navajo) and three advanced breeding lines (e.g., OP-8143 DH, OP-BN-72, SG-C 21215) had low titres of the virus after TuYV infection. This indicates a high level of resistance to TuYV in ‘Navajo’ or the newly developed breeding lines and the basis of resistance is probably different from R54 (as in ‘DK Temptation’). Furthermore, the greenhouse trials together with RT -qPCR-based virus titre analysis could be a cost-effective and efficient method to assess the level of resistance of a given genotype to TuYV infection compared to the field trials. However, further research is needed to identify the underlying mechanisms causing this difference in susceptibility.

Original language	English
Article number	2501
Pages (from-to)	1-14
Number of pages	14
Journal	Plants
Volume	12
Issue number	13
DOIs	https://doi.org/10.3390/plants12132501
Publication status	Published - Jul 2023

Bibliographical note

Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

aphids
doubled haploids (DH)
oilseed rape
resistance
RT-qPCR
TuYV
virus titre

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Cite this

@article{3ae32319854e4de7a23d936110f7b911,

title = "Evaluation of resistance of oilseed rape genotypes to turnip yellows virus",

abstract = "Turnip yellows virus (TuYV), is one of the most important pathogens of oilseed rape, which has caused enormous yield losses in all growing regions of the world in recent years. Therefore, there is a need for resistant varieties for sustainable crop protection. We have investigated the resistance of known varieties and newly developed advanced-breeding lines of oilseed rape to TuYV in greenhouse and field trials. We have analysed the TuYV titre of individual genotypes inoculated with the virus using viruliferous aphids Myzus persicae. The genotypes {\textquoteleft}DK Temptation{\textquoteright} and {\textquoteleft}Rescator{\textquoteright} had the lowest and highest virus titres, respectively, and were used as resistant and susceptible models for comparative analyses with other genotypes. In the greenhouse, the best results were obtained with the genotypes {\textquoteleft}OP-8143 DH{\textquoteright} (2.94 × 105 copies), OP-BN-72 (3.29 × 105 copies), {\textquoteleft}Navajo{\textquoteright} (3.58 × 105 copies) and {\textquoteleft}SG-C 21215{\textquoteright} (4.09 × 105 copies), which reached virus titres about 2 times higher than the minimum virus concentration measured in {\textquoteleft}DK Temptation{\textquoteright} (1.80 × 105 copies). In the field trials, the genotypes {\textquoteleft}Navajo{\textquoteright} (3.39 × 105 copies), {\textquoteleft}OP-8148 DH{\textquoteright} (4.44 × 105 copies), {\textquoteleft}SG-C 21215{\textquoteright} (6.80 × 105 copies) and OP-8480 (7.19 × 105 copies) had the lowest virus titres and reached about 3 times the virus titre of DK Temptation (2.54 × 105 copies). Both trials showed that at least two commercial varieties (e.g., DK Temptation, Navajo) and three advanced breeding lines (e.g., OP-8143 DH, OP-BN-72, SG-C 21215) had low titres of the virus after TuYV infection. This indicates a high level of resistance to TuYV in {\textquoteleft}Navajo{\textquoteright} or the newly developed breeding lines and the basis of resistance is probably different from R54 (as in {\textquoteleft}DK Temptation{\textquoteright}). Furthermore, the greenhouse trials together with RT -qPCR-based virus titre analysis could be a cost-effective and efficient method to assess the level of resistance of a given genotype to TuYV infection compared to the field trials. However, further research is needed to identify the underlying mechanisms causing this difference in susceptibility.",

keywords = "aphids, doubled haploids (DH), oilseed rape, resistance, RT-qPCR, TuYV, virus titre",

author = "Emad Ibrahim and Andrea Rychl{\'a} and Glenda Alquicer and Lucie Slav{\'i}kov{\'a} and Qi Peng and Miroslav Kl{\'i}ma and Viktor Vrbovsk{\'y} and Piotr Trebicki and Kundu, {Jiban Kumar}",

note = "Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher. ",

year = "2023",

month = jul,

doi = "10.3390/plants12132501",

language = "English",

volume = "12",

pages = "1--14",

journal = "Plants",

issn = "2223-7747",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "13",

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TY - JOUR

T1 - Evaluation of resistance of oilseed rape genotypes to turnip yellows virus

AU - Ibrahim, Emad

AU - Rychlá, Andrea

AU - Alquicer, Glenda

AU - Slavíková, Lucie

AU - Peng, Qi

AU - Klíma, Miroslav

AU - Vrbovský, Viktor

AU - Trebicki, Piotr

AU - Kundu, Jiban Kumar

N1 - Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2023/7

Y1 - 2023/7

N2 - Turnip yellows virus (TuYV), is one of the most important pathogens of oilseed rape, which has caused enormous yield losses in all growing regions of the world in recent years. Therefore, there is a need for resistant varieties for sustainable crop protection. We have investigated the resistance of known varieties and newly developed advanced-breeding lines of oilseed rape to TuYV in greenhouse and field trials. We have analysed the TuYV titre of individual genotypes inoculated with the virus using viruliferous aphids Myzus persicae. The genotypes ‘DK Temptation’ and ‘Rescator’ had the lowest and highest virus titres, respectively, and were used as resistant and susceptible models for comparative analyses with other genotypes. In the greenhouse, the best results were obtained with the genotypes ‘OP-8143 DH’ (2.94 × 105 copies), OP-BN-72 (3.29 × 105 copies), ‘Navajo’ (3.58 × 105 copies) and ‘SG-C 21215’ (4.09 × 105 copies), which reached virus titres about 2 times higher than the minimum virus concentration measured in ‘DK Temptation’ (1.80 × 105 copies). In the field trials, the genotypes ‘Navajo’ (3.39 × 105 copies), ‘OP-8148 DH’ (4.44 × 105 copies), ‘SG-C 21215’ (6.80 × 105 copies) and OP-8480 (7.19 × 105 copies) had the lowest virus titres and reached about 3 times the virus titre of DK Temptation (2.54 × 105 copies). Both trials showed that at least two commercial varieties (e.g., DK Temptation, Navajo) and three advanced breeding lines (e.g., OP-8143 DH, OP-BN-72, SG-C 21215) had low titres of the virus after TuYV infection. This indicates a high level of resistance to TuYV in ‘Navajo’ or the newly developed breeding lines and the basis of resistance is probably different from R54 (as in ‘DK Temptation’). Furthermore, the greenhouse trials together with RT -qPCR-based virus titre analysis could be a cost-effective and efficient method to assess the level of resistance of a given genotype to TuYV infection compared to the field trials. However, further research is needed to identify the underlying mechanisms causing this difference in susceptibility.

AB - Turnip yellows virus (TuYV), is one of the most important pathogens of oilseed rape, which has caused enormous yield losses in all growing regions of the world in recent years. Therefore, there is a need for resistant varieties for sustainable crop protection. We have investigated the resistance of known varieties and newly developed advanced-breeding lines of oilseed rape to TuYV in greenhouse and field trials. We have analysed the TuYV titre of individual genotypes inoculated with the virus using viruliferous aphids Myzus persicae. The genotypes ‘DK Temptation’ and ‘Rescator’ had the lowest and highest virus titres, respectively, and were used as resistant and susceptible models for comparative analyses with other genotypes. In the greenhouse, the best results were obtained with the genotypes ‘OP-8143 DH’ (2.94 × 105 copies), OP-BN-72 (3.29 × 105 copies), ‘Navajo’ (3.58 × 105 copies) and ‘SG-C 21215’ (4.09 × 105 copies), which reached virus titres about 2 times higher than the minimum virus concentration measured in ‘DK Temptation’ (1.80 × 105 copies). In the field trials, the genotypes ‘Navajo’ (3.39 × 105 copies), ‘OP-8148 DH’ (4.44 × 105 copies), ‘SG-C 21215’ (6.80 × 105 copies) and OP-8480 (7.19 × 105 copies) had the lowest virus titres and reached about 3 times the virus titre of DK Temptation (2.54 × 105 copies). Both trials showed that at least two commercial varieties (e.g., DK Temptation, Navajo) and three advanced breeding lines (e.g., OP-8143 DH, OP-BN-72, SG-C 21215) had low titres of the virus after TuYV infection. This indicates a high level of resistance to TuYV in ‘Navajo’ or the newly developed breeding lines and the basis of resistance is probably different from R54 (as in ‘DK Temptation’). Furthermore, the greenhouse trials together with RT -qPCR-based virus titre analysis could be a cost-effective and efficient method to assess the level of resistance of a given genotype to TuYV infection compared to the field trials. However, further research is needed to identify the underlying mechanisms causing this difference in susceptibility.

KW - aphids

KW - doubled haploids (DH)

KW - oilseed rape

KW - resistance

KW - RT-qPCR

KW - TuYV

KW - virus titre

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U2 - 10.3390/plants12132501

DO - 10.3390/plants12132501

M3 - Article

C2 - 37447062

AN - SCOPUS:85165211640

SN - 2223-7747

VL - 12

SP - 1

EP - 14

JO - Plants

JF - Plants

IS - 13

M1 - 2501

ER -

Evaluation of resistance of oilseed rape genotypes to turnip yellows virus

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