Evaluation of the In-Fusion™ cloning system for structural genomics

Current structural genomics studies are focused on high-throughput determination of protein structure. This requires large-scale cloning, expression, and purification of protein candidates. Clontech’s In-Fusion PCR cloning method provides superior results for high-volume cloning of a diverse range of genes. To illustrate this, the performance of Clontech’s In-Fusion method was compared against that of a competitor kit from Invitrogen for cloning gene cassettes. The results show that the In-Fusion cloning method can produce the desired number of clones in half the time. In-Fusion cloning is less susceptible to interference by impurities, and the lyophilized reactions eliminate freeze/thaw issues. Furthermore, the In-Fusion system can be tailored to many applications, since any vector may be used at any site of linearization, and will not add additional bases to the expression clone.