Evaluation of the In-Fusion™ cloning system for structural genomics

A. P. Guilfoyle, B. C. Mabbutt

Research output: Contribution to journalLetter

Abstract

Current structural genomics studies are focused on high-throughput determination of protein structure. This requires large-scale cloning, expression, and purification of protein candidates. Clontech’s In-Fusion PCR cloning method provides superior results for high-volume cloning of a diverse range of genes. To illustrate this, the performance of Clontech’s In-Fusion method was compared against that of a competitor kit from Invitrogen for cloning gene cassettes. The results show that the In-Fusion cloning method can produce the desired number of clones in half the time. In-Fusion cloning is less susceptible to interference by impurities, and the lyophilized reactions eliminate freeze/thaw issues. Furthermore, the In-Fusion system can be tailored to many applications, since any vector may be used at any site of linearization, and will not add additional bases to the expression clone.
Original languageEnglish
Pages (from-to)31-32
Number of pages2
JournalClontechniques
Volume22
Issue number4
Publication statusPublished - 2007

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