Evidence for a novel biological role for the multifunctional β-1,3-glucan binding protein in shrimp

Priscila Goncalves, Javier Vernal, Rafael Diego Rosa, Gloria Yepiz-Plascencia, Claudia Regina Batista de Souza, Margherita Anna Barracco, Luciane Maria Perazzolo*

*Corresponding author for this work

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

β-1,3-Glucan binding proteins (βGBPs) are soluble pattern recognition proteins/receptors that bind to β-1,3-glucans from fungi cell walls. In crustaceans, βGBPs are abundant plasmatic proteins produced by the hepatopancreas, and have been proved to play multiple biological functions. Here, we purified and characterized novel members of the βGBP family from the hemolymph of two Brazilian shrimps, Farfantepenaeus paulensis (FpβGBP) and Litopenaeus schmitti (LsβGBP). As observed for other crustacean species, FpβGBP and LsβGBP are monomeric proteins (∼100. kDa) able to enhance the activation of the prophenoloxidase system, a potent antimicrobial defense conserved in arthropods. More interestingly, we provided here evidence for a novel biological activity for shrimp βGBPs: the agglutination of fungal cells. Finally, we investigated the modulation of the βGBP gene in F. paulensis shrimps experimentally infected with a cognate fungal pathogen, Fusarium solani. From our expression data, βGBP gene is constitutively expressed in hepatopancreas and not modulated upon a non-lethal fungal infection. Herein, we have improved our knowledge about the βGBP family by the characterization of a novel biological role for this multifunctional protein in shrimp.

Original languageEnglish
Pages (from-to)363-367
Number of pages5
JournalMolecular Immunology
Volume51
Issue number3-4
DOIs
Publication statusPublished - Jul 2012

Keywords

  • βGBP
  • Lectin
  • Pattern recognition protein/receptor
  • Prophenoloxidase activating system
  • Shrimp immunity
  • Transcriptional response to fungal infection

Fingerprint Dive into the research topics of 'Evidence for a novel biological role for the multifunctional β-1,3-glucan binding protein in shrimp'. Together they form a unique fingerprint.

Cite this