Evolutionary engineering improves tolerance for replacement jet fuels in Saccharomyces cerevisiae

Timothy C.R. Brennan, Thomas C. Williams, Benjamin L. Schulz, Robin W. Palfreyman, Jens O. Krömer*, Lars K. Nielsen

*Corresponding author for this work

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Monoterpenes are liquid hydrocarbons with applications ranging from flavor and fragrance to replacement jet fuel. Their toxicity, however, presents a major challenge for microbial synthesis. Here we evolved limonene-tolerant Saccharomyces cerevisiae strains and sequenced six strains across the 200-generation evolutionary time course. Mutations were found in the tricalbin proteins Tcb2p and Tcb3p. Genomic reconstruction in the parent strain showed that truncation of a single protein (tTcb3p1-989), but not its complete deletion, was sufficient to recover the evolved phenotype improving limonene fitness 9-fold. tTcb3p1-989 increased tolerance toward two other monoterpenes (β-pinene and myrcene) 11- and 8-fold, respectively, and tolerance toward the biojet fuel blend AMJ-700t (10% cymene, 50% limonene, 40% farnesene) 4-fold. tTcb3p1-989 is the first example of successful engineering of phase tolerance and creates opportunities for production of the highly toxic C10 alkenes in yeast.

Original languageEnglish
Pages (from-to)3316-3325
Number of pages10
JournalApplied and Environmental Microbiology
Volume81
Issue number10
DOIs
Publication statusPublished - May 2015
Externally publishedYes

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