Examining cellular responses to kinase drug inhibition through phosphoproteome mapping of substrates

Daniel Bucio-Noble, Crystal Semaan, Mark P. Molloy*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

This chapter provides a detailed description for mass spectrometry-based phosphoproteomics analysis. We describe sample preparation, phosphopeptide enrichment, mass spectrometry acquisition, label-free data analysis and statistical analysis. This technique can be employed to characterize cell signaling networks, and is particularly useful to monitor cellular responses to kinase drug inhibitors.

Original languageEnglish
Title of host publicationSystems chemical biology
Subtitle of host publicationmethods and protocols
EditorsSlava Ziegler, Herbert Waldmann
Place of PublicationNew York
PublisherHumana Press
Chapter8
Pages141-152
Number of pages12
ISBN (Electronic)9781493988914
ISBN (Print)9781493988907
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1888
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Phosphoproteomics
  • Phosphopeptide enrichment
  • Mass spectrometry
  • Label-free

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