Exhaled breath condensate biomarkers in asbestos-related lung disorders

Sharron Chow; Charlotte Campbell; Alessandra Sandrini; Paul S. Thomas; Anthony R. Johnson; Deborah H. Yates

doi:10.1016/j.rmed.2009.04.009

Exhaled breath condensate biomarkers in asbestos-related lung disorders

Sharron Chow, Charlotte Campbell, Alessandra Sandrini, Paul S. Thomas, Anthony R. Johnson, Deborah H. Yates^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Objectives: Asbestos induces generation of reactive oxygen and nitrogen species in laboratory studies. Several such species can be measured non-invasively in humans in exhaled breath condensate (EBC) but few have been evaluated. This study aimed to assess oxidative stress and lung inflammation in vivo.

Methods: Eighty six men were studied: sixty subjects with asbestos-related disorders (asbestosis: 18, diffuse pleural thickening (DPT): 16, pleural plaques (PPs): 26) and twenty six age- and gender-matched normal individuals.

Results: Subjects with asbestosis had raised EBC markers of oxidative stress compared with normal controls [8-isoprostane (geometric mean (95% CI) 0.51 (0.17-1.51) vs 0.07 (0.04-0.13) ng/ml, p <0.01); hydrogen peroxide (13.68 (8.63-21.68) vs 5.89 (3.99-8.69) mu M, p <0.05), as well as increased EBC total protein (17.27 (10.57-28.23) vs 7.62 (5.13-11.34) mu g/ml, p <0.05), and fractional exhaled nitric oxide (mean +/- SD) (9.67 +/- 3.26 vs 7.57 +/- 1.89 ppb; p <0.05). EBC pH was lower in subjects with asbestosis compared with subjects with DPT (7.26 +/- 0.31 vs 7.53 +/- 0.24; p <0.05). There were no significant differences in exhaled carbon monoxide, EBC total nitrogen oxides and 3-nitrotyrosine between any of the asbestos-related disorders, or between these and controls.

Conclusion: In asbestos-related disorders, markers of inflammation and oxidative stress are significantly elevated in subjects with asbestosis compared with healthy individuals but not in pleural. diseases. (C) 2009 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	1091-1097
Number of pages	7
Journal	Respiratory Medicine
Volume	103
Issue number	8
DOIs	https://doi.org/10.1016/j.rmed.2009.04.009
Publication status	Published - Aug 2009
Externally published	Yes

Keywords

Exhaled breath condensate
Asbestos
Exhaled nitric oxide
Oxidative stress
NITRIC-OXIDE CONCENTRATION
AIRWAY ACIDIFICATION
ASTHMATIC-PATIENTS
OXIDATIVE STRESS
CYSTIC-FIBROSIS
PH
8-ISOPROSTANE
FIBERS
MESOTHELIOMA
INFLAMMATION

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10.1016/j.rmed.2009.04.009

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@article{6d81a7b7aba24400afdadcdae5ce48d1,

title = "Exhaled breath condensate biomarkers in asbestos-related lung disorders",

abstract = "Objectives: Asbestos induces generation of reactive oxygen and nitrogen species in laboratory studies. Several such species can be measured non-invasively in humans in exhaled breath condensate (EBC) but few have been evaluated. This study aimed to assess oxidative stress and lung inflammation in vivo.Methods: Eighty six men were studied: sixty subjects with asbestos-related disorders (asbestosis: 18, diffuse pleural thickening (DPT): 16, pleural plaques (PPs): 26) and twenty six age- and gender-matched normal individuals.Results: Subjects with asbestosis had raised EBC markers of oxidative stress compared with normal controls [8-isoprostane (geometric mean (95% CI) 0.51 (0.17-1.51) vs 0.07 (0.04-0.13) ng/ml, p <0.01); hydrogen peroxide (13.68 (8.63-21.68) vs 5.89 (3.99-8.69) mu M, p <0.05), as well as increased EBC total protein (17.27 (10.57-28.23) vs 7.62 (5.13-11.34) mu g/ml, p <0.05), and fractional exhaled nitric oxide (mean +/- SD) (9.67 +/- 3.26 vs 7.57 +/- 1.89 ppb; p <0.05). EBC pH was lower in subjects with asbestosis compared with subjects with DPT (7.26 +/- 0.31 vs 7.53 +/- 0.24; p <0.05). There were no significant differences in exhaled carbon monoxide, EBC total nitrogen oxides and 3-nitrotyrosine between any of the asbestos-related disorders, or between these and controls.Conclusion: In asbestos-related disorders, markers of inflammation and oxidative stress are significantly elevated in subjects with asbestosis compared with healthy individuals but not in pleural. diseases. (C) 2009 Elsevier Ltd. All rights reserved.",

keywords = "Exhaled breath condensate, Asbestos, Exhaled nitric oxide, Oxidative stress, NITRIC-OXIDE CONCENTRATION, AIRWAY ACIDIFICATION, ASTHMATIC-PATIENTS, OXIDATIVE STRESS, CYSTIC-FIBROSIS, PH, 8-ISOPROSTANE, FIBERS, MESOTHELIOMA, INFLAMMATION",

author = "Sharron Chow and Charlotte Campbell and Alessandra Sandrini and Thomas, {Paul S.} and Johnson, {Anthony R.} and Yates, {Deborah H.}",

year = "2009",

month = aug,

doi = "10.1016/j.rmed.2009.04.009",

language = "English",

volume = "103",

pages = "1091--1097",

journal = "Respiratory Medicine",

issn = "0954-6111",

publisher = "W. B. Saunders",

number = "8",

}

TY - JOUR

T1 - Exhaled breath condensate biomarkers in asbestos-related lung disorders

AU - Chow, Sharron

AU - Campbell, Charlotte

AU - Sandrini, Alessandra

AU - Thomas, Paul S.

AU - Johnson, Anthony R.

AU - Yates, Deborah H.

PY - 2009/8

Y1 - 2009/8

N2 - Objectives: Asbestos induces generation of reactive oxygen and nitrogen species in laboratory studies. Several such species can be measured non-invasively in humans in exhaled breath condensate (EBC) but few have been evaluated. This study aimed to assess oxidative stress and lung inflammation in vivo.Methods: Eighty six men were studied: sixty subjects with asbestos-related disorders (asbestosis: 18, diffuse pleural thickening (DPT): 16, pleural plaques (PPs): 26) and twenty six age- and gender-matched normal individuals.Results: Subjects with asbestosis had raised EBC markers of oxidative stress compared with normal controls [8-isoprostane (geometric mean (95% CI) 0.51 (0.17-1.51) vs 0.07 (0.04-0.13) ng/ml, p <0.01); hydrogen peroxide (13.68 (8.63-21.68) vs 5.89 (3.99-8.69) mu M, p <0.05), as well as increased EBC total protein (17.27 (10.57-28.23) vs 7.62 (5.13-11.34) mu g/ml, p <0.05), and fractional exhaled nitric oxide (mean +/- SD) (9.67 +/- 3.26 vs 7.57 +/- 1.89 ppb; p <0.05). EBC pH was lower in subjects with asbestosis compared with subjects with DPT (7.26 +/- 0.31 vs 7.53 +/- 0.24; p <0.05). There were no significant differences in exhaled carbon monoxide, EBC total nitrogen oxides and 3-nitrotyrosine between any of the asbestos-related disorders, or between these and controls.Conclusion: In asbestos-related disorders, markers of inflammation and oxidative stress are significantly elevated in subjects with asbestosis compared with healthy individuals but not in pleural. diseases. (C) 2009 Elsevier Ltd. All rights reserved.

AB - Objectives: Asbestos induces generation of reactive oxygen and nitrogen species in laboratory studies. Several such species can be measured non-invasively in humans in exhaled breath condensate (EBC) but few have been evaluated. This study aimed to assess oxidative stress and lung inflammation in vivo.Methods: Eighty six men were studied: sixty subjects with asbestos-related disorders (asbestosis: 18, diffuse pleural thickening (DPT): 16, pleural plaques (PPs): 26) and twenty six age- and gender-matched normal individuals.Results: Subjects with asbestosis had raised EBC markers of oxidative stress compared with normal controls [8-isoprostane (geometric mean (95% CI) 0.51 (0.17-1.51) vs 0.07 (0.04-0.13) ng/ml, p <0.01); hydrogen peroxide (13.68 (8.63-21.68) vs 5.89 (3.99-8.69) mu M, p <0.05), as well as increased EBC total protein (17.27 (10.57-28.23) vs 7.62 (5.13-11.34) mu g/ml, p <0.05), and fractional exhaled nitric oxide (mean +/- SD) (9.67 +/- 3.26 vs 7.57 +/- 1.89 ppb; p <0.05). EBC pH was lower in subjects with asbestosis compared with subjects with DPT (7.26 +/- 0.31 vs 7.53 +/- 0.24; p <0.05). There were no significant differences in exhaled carbon monoxide, EBC total nitrogen oxides and 3-nitrotyrosine between any of the asbestos-related disorders, or between these and controls.Conclusion: In asbestos-related disorders, markers of inflammation and oxidative stress are significantly elevated in subjects with asbestosis compared with healthy individuals but not in pleural. diseases. (C) 2009 Elsevier Ltd. All rights reserved.

KW - Exhaled breath condensate

KW - Asbestos

KW - Exhaled nitric oxide

KW - Oxidative stress

KW - NITRIC-OXIDE CONCENTRATION

KW - AIRWAY ACIDIFICATION

KW - ASTHMATIC-PATIENTS

KW - OXIDATIVE STRESS

KW - CYSTIC-FIBROSIS

KW - PH

KW - 8-ISOPROSTANE

KW - FIBERS

KW - MESOTHELIOMA

KW - INFLAMMATION

U2 - 10.1016/j.rmed.2009.04.009

DO - 10.1016/j.rmed.2009.04.009

M3 - Article

C2 - 19520561

SN - 0954-6111

VL - 103

SP - 1091

EP - 1097

JO - Respiratory Medicine

JF - Respiratory Medicine

IS - 8

ER -

Exhaled breath condensate biomarkers in asbestos-related lung disorders

Abstract

Keywords

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