This study investigated the feasibility of forming vascular anastomoses by use of argon laser tissue fusion and absorbable, monofilament polydimethylsiloxane guy sutures. In initial animal studies femoral arteriovenous fistulas approximately 1.5 cm in length were created bilaterally in each of 10 dogs and were studied histologically at 2, 4, 8, 16, and 24 weeks (two animals in each interval). In each animal, one anastomosis (control) was formed with continuous 6-0 polypropylene suture, and the contralateral anastomosis (experimental) was performed with an argon laser (0.5 watt, 5 to 7 minutes exposure, energy fluence 1100 to 1500 joules/cm2 per 1 cm length) with stay sutures of 5-0 polydimethylsiloxane at 0.5 to 0.65 cm intervals. At removal, all anastomoses were patent without hematomas, aneurysms, or luminal narrowing. Histologic examination at 2 to 16 weeks demonstrated resorption of the biodegradable suture material by a local inflammatory reaction. By 24 weeks, laser-fused specimens had no evidence of suture material at the anastomotic line, and healing consisted of a bond between artery and vein wall tissues. Control suture specimens at the same intervals exhibited an organized fibrous tissue response to the suture. Clinical adaptability of this technology has subsequently been evaluated in five patients at 10 to 27 months (21.6 ± 5.8) by physical examination and duplex scanning and demonstrate no evidence of abnormal healing. This study establishes the experimental and preliminary clinical feasibility of laser-fused anastomoses aligned by biodegradable guy sutures and supports further investigation and refinement of the technique.