Expression and processing of a major xylanase (XYN2) from the thermophilic fungus Humicola grisea var. thermoidea in Trichoderma reesei

F. P. De Faria; V. S J Te'O; P. L. Bergquist; M. O. Azevedo; K. M H Nevalainen

doi:10.1046/j.1472-765x.2002.01057.x

Expression and processing of a major xylanase (XYN2) from the thermophilic fungus Humicola grisea var. thermoidea in Trichoderma reesei

F. P. De Faria, V. S J Te'O, P. L. Bergquist, M. O. Azevedo, K. M H Nevalainen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

Aims: To express a gene encoding a heterologous fungal xylanase in Trichoderma reesei. Methods and Results: Humicola grisea xylanase 2 (xyn2) cDNA was expressed in Trichoderma reesei under the main cellobiohydrolase I (cbh1) promoter (i) as a fusion to the cellobiohydrolase I (CBHI) secretion signal and (ii) the mature CBHI core-linker. The recombinant xylanase (HXYN2) was secreted into the cultivation medium and processed in a similar fashion to the endogenous T. reesei xylanases, resulting in an active enzyme. Conclusions, Significance and Impact of the Study: HXYN2 was successfully processed in T. reesei. Composition of the culture medium affected the HXYN2 yields, favouring Avicel-lactose as a carbon source. Best yields (about 0.5 g l^-1) in shake flask cultivations were obtained from a transformant where xyn2 was fused directly to the CBHI secretion signal.

Original language	English
Pages (from-to)	119-123
Number of pages	5
Journal	Letters in Applied Microbiology
Volume	34
Issue number	2
DOIs	https://doi.org/10.1046/j.1472-765x.2002.01057.x
Publication status	Published - 2002

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title = "Expression and processing of a major xylanase (XYN2) from the thermophilic fungus Humicola grisea var. thermoidea in Trichoderma reesei",

abstract = "Aims: To express a gene encoding a heterologous fungal xylanase in Trichoderma reesei. Methods and Results: Humicola grisea xylanase 2 (xyn2) cDNA was expressed in Trichoderma reesei under the main cellobiohydrolase I (cbh1) promoter (i) as a fusion to the cellobiohydrolase I (CBHI) secretion signal and (ii) the mature CBHI core-linker. The recombinant xylanase (HXYN2) was secreted into the cultivation medium and processed in a similar fashion to the endogenous T. reesei xylanases, resulting in an active enzyme. Conclusions, Significance and Impact of the Study: HXYN2 was successfully processed in T. reesei. Composition of the culture medium affected the HXYN2 yields, favouring Avicel-lactose as a carbon source. Best yields (about 0.5 g l-1) in shake flask cultivations were obtained from a transformant where xyn2 was fused directly to the CBHI secretion signal.",

author = "{De Faria}, {F. P.} and Te'O, {V. S J} and Bergquist, {P. L.} and Azevedo, {M. O.} and Nevalainen, {K. M H}",

year = "2002",

doi = "10.1046/j.1472-765x.2002.01057.x",

language = "English",

volume = "34",

pages = "119--123",

journal = "Letters in Applied Microbiology",

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T1 - Expression and processing of a major xylanase (XYN2) from the thermophilic fungus Humicola grisea var. thermoidea in Trichoderma reesei

AU - De Faria, F. P.

AU - Te'O, V. S J

AU - Bergquist, P. L.

AU - Azevedo, M. O.

AU - Nevalainen, K. M H

PY - 2002

Y1 - 2002

N2 - Aims: To express a gene encoding a heterologous fungal xylanase in Trichoderma reesei. Methods and Results: Humicola grisea xylanase 2 (xyn2) cDNA was expressed in Trichoderma reesei under the main cellobiohydrolase I (cbh1) promoter (i) as a fusion to the cellobiohydrolase I (CBHI) secretion signal and (ii) the mature CBHI core-linker. The recombinant xylanase (HXYN2) was secreted into the cultivation medium and processed in a similar fashion to the endogenous T. reesei xylanases, resulting in an active enzyme. Conclusions, Significance and Impact of the Study: HXYN2 was successfully processed in T. reesei. Composition of the culture medium affected the HXYN2 yields, favouring Avicel-lactose as a carbon source. Best yields (about 0.5 g l-1) in shake flask cultivations were obtained from a transformant where xyn2 was fused directly to the CBHI secretion signal.

AB - Aims: To express a gene encoding a heterologous fungal xylanase in Trichoderma reesei. Methods and Results: Humicola grisea xylanase 2 (xyn2) cDNA was expressed in Trichoderma reesei under the main cellobiohydrolase I (cbh1) promoter (i) as a fusion to the cellobiohydrolase I (CBHI) secretion signal and (ii) the mature CBHI core-linker. The recombinant xylanase (HXYN2) was secreted into the cultivation medium and processed in a similar fashion to the endogenous T. reesei xylanases, resulting in an active enzyme. Conclusions, Significance and Impact of the Study: HXYN2 was successfully processed in T. reesei. Composition of the culture medium affected the HXYN2 yields, favouring Avicel-lactose as a carbon source. Best yields (about 0.5 g l-1) in shake flask cultivations were obtained from a transformant where xyn2 was fused directly to the CBHI secretion signal.

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DO - 10.1046/j.1472-765x.2002.01057.x

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AN - SCOPUS:0036151166

SN - 0266-8254

VL - 34

SP - 119

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JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

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ER -

Expression and processing of a major xylanase (XYN2) from the thermophilic fungus Humicola grisea var. thermoidea in Trichoderma reesei

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