Expression and secretion of Bacillus amyloliquefaciens α-amylase by using the yeast pheromone α-factor promoter and leader sequence in Saccharomyces cerevisiae

V. J. Southgate, A. J C Steyn, I. S. Pretorius, H. J J Van Vuuren*

*Corresponding author for this work

Research output: Contribution to journalComment/opinion

16 Citations (Scopus)

Abstract

Replacement of the regulatory and secretory signals of the α-amylase gene (AMY) from Bacillus amyloliquefaciens with the complete yeast pheromone α- factor prepro region (MFα1(P)) resulted in increased levels of extracellular α-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala) 2 peptide from the MFα1 spacer region (Lys-Arg-Glu-Ala-Glu- Ala) yielded decreased levels of extracellular α-amylase.

Original languageEnglish
Pages (from-to)1253-1258
Number of pages6
JournalApplied and Environmental Microbiology
Volume59
Issue number4
Publication statusPublished - 1993
Externally publishedYes

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