Expression and secretion of Bacillus amyloliquefaciens α-amylase by using the yeast pheromone α-factor promoter and leader sequence in Saccharomyces cerevisiae

V. J. Southgate; A. J C Steyn; I. S. Pretorius; H. J J Van Vuuren

Expression and secretion of Bacillus amyloliquefaciens α-amylase by using the yeast pheromone α-factor promoter and leader sequence in Saccharomyces cerevisiae

V. J. Southgate, A. J C Steyn, I. S. Pretorius, H. J J Van Vuuren^*

^*Corresponding author for this work

Research output: Contribution to journal › Comment/opinion › peer-review

16 Citations (Scopus)

Abstract

Replacement of the regulatory and secretory signals of the α-amylase gene (AMY) from Bacillus amyloliquefaciens with the complete yeast pheromone α- factor prepro region (MFα1(P)) resulted in increased levels of extracellular α-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala) ₂ peptide from the MFα1 spacer region (Lys-Arg-Glu-Ala-Glu- Ala) yielded decreased levels of extracellular α-amylase.

Original language	English
Pages (from-to)	1253-1258
Number of pages	6
Journal	Applied and Environmental Microbiology
Volume	59
Issue number	4
Publication status	Published - 1993
Externally published	Yes

Cite this

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title = "Expression and secretion of Bacillus amyloliquefaciens α-amylase by using the yeast pheromone α-factor promoter and leader sequence in Saccharomyces cerevisiae",

abstract = "Replacement of the regulatory and secretory signals of the α-amylase gene (AMY) from Bacillus amyloliquefaciens with the complete yeast pheromone α- factor prepro region (MFα1(P)) resulted in increased levels of extracellular α-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala) 2 peptide from the MFα1 spacer region (Lys-Arg-Glu-Ala-Glu- Ala) yielded decreased levels of extracellular α-amylase.",

author = "Southgate, {V. J.} and Steyn, {A. J C} and Pretorius, {I. S.} and {Van Vuuren}, {H. J J}",

year = "1993",

language = "English",

volume = "59",

pages = "1253--1258",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "4",

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Expression and secretion of Bacillus amyloliquefaciens α-amylase by using the yeast pheromone α-factor promoter and leader sequence in Saccharomyces cerevisiae. / Southgate, V. J.; Steyn, A. J C; Pretorius, I. S. et al.
In: Applied and Environmental Microbiology, Vol. 59, No. 4, 1993, p. 1253-1258.

Research output: Contribution to journal › Comment/opinion › peer-review

TY - JOUR

T1 - Expression and secretion of Bacillus amyloliquefaciens α-amylase by using the yeast pheromone α-factor promoter and leader sequence in Saccharomyces cerevisiae

AU - Southgate, V. J.

AU - Steyn, A. J C

AU - Pretorius, I. S.

AU - Van Vuuren, H. J J

PY - 1993

Y1 - 1993

N2 - Replacement of the regulatory and secretory signals of the α-amylase gene (AMY) from Bacillus amyloliquefaciens with the complete yeast pheromone α- factor prepro region (MFα1(P)) resulted in increased levels of extracellular α-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala) 2 peptide from the MFα1 spacer region (Lys-Arg-Glu-Ala-Glu- Ala) yielded decreased levels of extracellular α-amylase.

AB - Replacement of the regulatory and secretory signals of the α-amylase gene (AMY) from Bacillus amyloliquefaciens with the complete yeast pheromone α- factor prepro region (MFα1(P)) resulted in increased levels of extracellular α-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala) 2 peptide from the MFα1 spacer region (Lys-Arg-Glu-Ala-Glu- Ala) yielded decreased levels of extracellular α-amylase.

UR - http://www.scopus.com/inward/record.url?scp=0027502427&partnerID=8YFLogxK

M3 - Comment/opinion

C2 - 8476297

AN - SCOPUS:0027502427

SN - 0099-2240

VL - 59

SP - 1253

EP - 1258

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 4

ER -

Expression and secretion of Bacillus amyloliquefaciens α-amylase by using the yeast pheromone α-factor promoter and leader sequence in Saccharomyces cerevisiae

Abstract

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