Expression of a shark antibody using Trichoderma reesei as a heterologous host

S. Mohammed*, V. Te'o, Stewart Nuttall, H. Nevalainen

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

Abstract

The increasing demand on the manufacture of therapeutic proteins has led to the use of microbial expression systems for the heterologous production of the desired protein. The filamentous fungus Trichoderma reesei has been proven to be a powerful expression system for the production of various heterologous proteins and their secretion to the culture medium (Nevalainen et al., 2005). In this study, we have used a low protease strain of T. reesei for the production of a shark antibody fragment variable region (V-NAR) of an antibody molecule called I(g)NAR (Immunoglobulin new antigen receptor) from the Wobbegong shark (Orectolobus maculatus). These single antibody variable domains are used as binding proteins and possess a number of applications in human medicine due to their relatively small structure and stability (Nuttall et al., 2002, 2003).

Original languageEnglish
Title of host publicationProceedings of the 8th International Mycological Congress
EditorsWieland Meyer, Ceri Pearce
Place of PublicationBologna
PublisherMEDIMOND S R L
Pages91-96
Number of pages6
ISBN (Print)9788875872762
Publication statusPublished - 2006
Event8th International Mycological Congress of the International-Mycological-Association (IMC8) - Cairns, Australia
Duration: 20 Aug 200625 Aug 2006

Conference

Conference8th International Mycological Congress of the International-Mycological-Association (IMC8)
CountryAustralia
CityCairns
Period20/08/0625/08/06

Keywords

  • FRAGMENTS
  • LACCASE
  • ENZYME

Cite this

Mohammed, S., Te'o, V., Nuttall, S., & Nevalainen, H. (2006). Expression of a shark antibody using Trichoderma reesei as a heterologous host. In W. Meyer, & C. Pearce (Eds.), Proceedings of the 8th International Mycological Congress (pp. 91-96). [S24IS3 - 0834] Bologna: MEDIMOND S R L.