Expression of barley endopeptidase B in Trichoderma reesei

Ritva Saarelainen, Arja Mäntylä, Helena Nevalainen, Pirkko Suominen*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    The gene for barley endopeptidase B (EPB) has been expressed in the filamentous fungus Trichoderma reesei from the cbh1 promoter. The EPB signal sequence allowed secretion of over 90% of the recombinant protein. Yields reached about 500 mg of immunoreactive protein per liter and exceeded values for any other protein derived from a higher eukaryotic organism produced in T. reesei.

    Original languageEnglish
    Pages (from-to)4938-4940
    Number of pages3
    JournalApplied and Environmental Microbiology
    Volume63
    Issue number12
    Publication statusPublished - Dec 1997

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