Expression of growth factors in Dictyostelium discoideum

S. Asgari*, S. Arun, M. B. Slade, J. Marshall, K. L. Williams, J. F. Wheldrake

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Growth factors and their binding proteins are important proteins regulating mammalian cell proliferation and differentiation so there is considerable interest in producing them as recombinant proteins, especially in hosts that do not already produce a complex mixture of growth factors. Many growth factors require post-translational modifications making them unsuitable for production in Escherichia coli or other prokaryotes. Since several expression vector systems have been recently developed for foreign protein production in the cellular slime mould, Dictyostelium discoideum, we attempted to use two of these systems to express human insulin-like growth factor binding protein 6 (hlGFBP6) and bovine beta-cellulin (bBTC) as secreted proteins. Although both proteins were successfully produced in stably transformed amoebae, no secretion was detected in spite of several attempts to facilitate this occurring.

    Original languageEnglish
    Pages (from-to)491-497
    Number of pages7
    JournalJournal of Molecular Microbiology and Biotechnology
    Volume3
    Issue number3
    Publication statusPublished - 2001

    Fingerprint

    Dive into the research topics of 'Expression of growth factors in Dictyostelium discoideum'. Together they form a unique fingerprint.

    Cite this