Expression of leucine genes from an extremely thermophilic bacterium in Escherichia coli

Julie E. Croft, Donald R. Love, Peter L. Bergquist*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

The organisation of the leucine genes in Thermus thermophilus HB8 was analysed by examining the ability of recombinant DNAs to complement Escherichia coli mutations. The arrangement of the genes is different from that in the mesophilic bacteria E. coli and Salmonella typhimurium. The promoter responsible for the expression of the leuB, leuC and leuD genes of Thermus HB8 in E. coli was identified. The sequence of Thermus DNA containing this promoter revealed structural similarities to the promoter and attenuator regions of the E. coli leucine operon.

Original languageEnglish
Pages (from-to)490-497
Number of pages8
JournalMGG Molecular & General Genetics
Volume210
Issue number3
DOIs
Publication statusPublished - Dec 1987
Externally publishedYes

Keywords

  • Complementation
  • Leucine operon
  • Promoter
  • Thermus

Fingerprint Dive into the research topics of 'Expression of leucine genes from an extremely thermophilic bacterium in Escherichia coli'. Together they form a unique fingerprint.

Cite this