Expression of leucine genes from an extremely thermophilic bacterium in Escherichia coli

Julie E. Croft; Donald R. Love; Peter L. Bergquist

doi:10.1007/BF00327202

Expression of leucine genes from an extremely thermophilic bacterium in Escherichia coli

Julie E. Croft, Donald R. Love, Peter L. Bergquist^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

The organisation of the leucine genes in Thermus thermophilus HB8 was analysed by examining the ability of recombinant DNAs to complement Escherichia coli mutations. The arrangement of the genes is different from that in the mesophilic bacteria E. coli and Salmonella typhimurium. The promoter responsible for the expression of the leuB, leuC and leuD genes of Thermus HB8 in E. coli was identified. The sequence of Thermus DNA containing this promoter revealed structural similarities to the promoter and attenuator regions of the E. coli leucine operon.

Original language	English
Pages (from-to)	490-497
Number of pages	8
Journal	MGG Molecular & General Genetics
Volume	210
Issue number	3
DOIs	https://doi.org/10.1007/BF00327202
Publication status	Published - Dec 1987
Externally published	Yes

Keywords

Complementation
Leucine operon
Promoter
Thermus

Access to Document

10.1007/BF00327202

Cite this

@article{daf041d0325b48c9abb80a5f77ec5f24,

title = "Expression of leucine genes from an extremely thermophilic bacterium in Escherichia coli",

abstract = "The organisation of the leucine genes in Thermus thermophilus HB8 was analysed by examining the ability of recombinant DNAs to complement Escherichia coli mutations. The arrangement of the genes is different from that in the mesophilic bacteria E. coli and Salmonella typhimurium. The promoter responsible for the expression of the leuB, leuC and leuD genes of Thermus HB8 in E. coli was identified. The sequence of Thermus DNA containing this promoter revealed structural similarities to the promoter and attenuator regions of the E. coli leucine operon.",

keywords = "Complementation, Leucine operon, Promoter, Thermus",

author = "Croft, \{Julie E.\} and Love, \{Donald R.\} and Bergquist, \{Peter L.\}",

year = "1987",

month = dec,

doi = "10.1007/BF00327202",

language = "English",

volume = "210",

pages = "490--497",

journal = "MGG Molecular \& General Genetics",

issn = "0026-8925",

publisher = "Springer, Springer Nature",

number = "3",

}

TY - JOUR

T1 - Expression of leucine genes from an extremely thermophilic bacterium in Escherichia coli

AU - Croft, Julie E.

AU - Love, Donald R.

AU - Bergquist, Peter L.

PY - 1987/12

Y1 - 1987/12

N2 - The organisation of the leucine genes in Thermus thermophilus HB8 was analysed by examining the ability of recombinant DNAs to complement Escherichia coli mutations. The arrangement of the genes is different from that in the mesophilic bacteria E. coli and Salmonella typhimurium. The promoter responsible for the expression of the leuB, leuC and leuD genes of Thermus HB8 in E. coli was identified. The sequence of Thermus DNA containing this promoter revealed structural similarities to the promoter and attenuator regions of the E. coli leucine operon.

AB - The organisation of the leucine genes in Thermus thermophilus HB8 was analysed by examining the ability of recombinant DNAs to complement Escherichia coli mutations. The arrangement of the genes is different from that in the mesophilic bacteria E. coli and Salmonella typhimurium. The promoter responsible for the expression of the leuB, leuC and leuD genes of Thermus HB8 in E. coli was identified. The sequence of Thermus DNA containing this promoter revealed structural similarities to the promoter and attenuator regions of the E. coli leucine operon.

KW - Complementation

KW - Leucine operon

KW - Promoter

KW - Thermus

UR - https://www.scopus.com/pages/publications/0023504697

U2 - 10.1007/BF00327202

DO - 10.1007/BF00327202

M3 - Article

C2 - 3323845

AN - SCOPUS:0023504697

SN - 0026-8925

VL - 210

SP - 490

EP - 497

JO - MGG Molecular & General Genetics

JF - MGG Molecular & General Genetics

IS - 3

ER -

Expression of leucine genes from an extremely thermophilic bacterium in Escherichia coli

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this