Expression of the Ruminococcus flavefaciens cellodextrinase gene in Saccharomyces cerevisiae

Pierre van Rensburg*, Willem H. van Zyl, Isak S. Pretorius

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A recombinant strain of Saccharomyces cerevisiae secreting bacterial cellodextrinase was constructed. The Ruminococcus flavefaciens cellodextrinase gene (celA) was inserted between a yeast expression-secretion cassette and yeast gene terminator, and cloned into a yeast-centromeric shuttle vector. Enzyme assays revealed growth-associated production of biologically active cellodextrinase by S. cerevisiae transformants.

Original languageEnglish
Pages (from-to)481-486
Number of pages6
JournalBiotechnology Letters
Volume17
Issue number5
DOIs
Publication statusPublished - May 1995
Externally publishedYes

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