Expression of the Ruminococcus flavefaciens cellodextrinase gene in Saccharomyces cerevisiae

Pierre van Rensburg; Willem H. van Zyl; Isak S. Pretorius

doi:10.1007/BF00132014

Expression of the Ruminococcus flavefaciens cellodextrinase gene in Saccharomyces cerevisiae

Pierre van Rensburg^*, Willem H. van Zyl, Isak S. Pretorius

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A recombinant strain of Saccharomyces cerevisiae secreting bacterial cellodextrinase was constructed. The Ruminococcus flavefaciens cellodextrinase gene (celA) was inserted between a yeast expression-secretion cassette and yeast gene terminator, and cloned into a yeast-centromeric shuttle vector. Enzyme assays revealed growth-associated production of biologically active cellodextrinase by S. cerevisiae transformants.

Original language	English
Pages (from-to)	481-486
Number of pages	6
Journal	Biotechnology Letters
Volume	17
Issue number	5
DOIs	https://doi.org/10.1007/BF00132014
Publication status	Published - May 1995
Externally published	Yes

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@article{cc8788760228459f933f0374f8a2aa64,

title = "Expression of the Ruminococcus flavefaciens cellodextrinase gene in Saccharomyces cerevisiae",

abstract = "A recombinant strain of Saccharomyces cerevisiae secreting bacterial cellodextrinase was constructed. The Ruminococcus flavefaciens cellodextrinase gene (celA) was inserted between a yeast expression-secretion cassette and yeast gene terminator, and cloned into a yeast-centromeric shuttle vector. Enzyme assays revealed growth-associated production of biologically active cellodextrinase by S. cerevisiae transformants.",

author = "{van Rensburg}, Pierre and {van Zyl}, {Willem H.} and Pretorius, {Isak S.}",

year = "1995",

month = may,

doi = "10.1007/BF00132014",

language = "English",

volume = "17",

pages = "481--486",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer, Springer Nature",

number = "5",

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TY - JOUR

T1 - Expression of the Ruminococcus flavefaciens cellodextrinase gene in Saccharomyces cerevisiae

AU - van Rensburg, Pierre

AU - van Zyl, Willem H.

AU - Pretorius, Isak S.

PY - 1995/5

Y1 - 1995/5

N2 - A recombinant strain of Saccharomyces cerevisiae secreting bacterial cellodextrinase was constructed. The Ruminococcus flavefaciens cellodextrinase gene (celA) was inserted between a yeast expression-secretion cassette and yeast gene terminator, and cloned into a yeast-centromeric shuttle vector. Enzyme assays revealed growth-associated production of biologically active cellodextrinase by S. cerevisiae transformants.

AB - A recombinant strain of Saccharomyces cerevisiae secreting bacterial cellodextrinase was constructed. The Ruminococcus flavefaciens cellodextrinase gene (celA) was inserted between a yeast expression-secretion cassette and yeast gene terminator, and cloned into a yeast-centromeric shuttle vector. Enzyme assays revealed growth-associated production of biologically active cellodextrinase by S. cerevisiae transformants.

UR - http://www.scopus.com/inward/record.url?scp=0029004820&partnerID=8YFLogxK

U2 - 10.1007/BF00132014

DO - 10.1007/BF00132014

M3 - Article

AN - SCOPUS:0029004820

SN - 0141-5492

VL - 17

SP - 481

EP - 486

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 5

ER -

Expression of the Ruminococcus flavefaciens cellodextrinase gene in Saccharomyces cerevisiae

Abstract

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