Extending the known range of glycerol ether lipids in the environment

Structural assignments based on tandem mass spectral fragmentation patterns

Xiao Lei Liu*, Roger E. Summons, Kai Uwe Hinrichs

*Corresponding author for this work

    Research output: Contribution to journalArticle

    67 Citations (Scopus)

    Abstract

    Ratione: Glycerol-based alkyl ether lipids are ubiquitous components in marine sediments. In order to explore their structural diversity and biological sources, marine sediment samples from diverse environments were analyzed and the mass spectra of widely distributed, novel glycerol di- and tetraethers were examined systematically. Methods: Lipid extracts of twelve globally distributed marine subsurface sediments were analyzed by atmospheric pressure chemical ionization mass spectrometry (APCI-MS). Tandem mass (MS/MS) spectra of compounds were obtained with a quadrupole time-of-flight (qTOF) mass spectrometer. RESULTS: In addition to the well-established isoprenoidal glycerol dialkyl glycerol tetraether (isoprenoidal GDGT) and branched GDGT, suites of novel lipids were detected in all studied samples. These lipids include the following classes of tentatively identified compounds: isoprenoidal glycerol dialkanol diether (isoprenoidal GDD), hydroxylated isoprenoidal GDGT (OH-GDGT), hybrid isoprenoidal/branched GDGT (IB-GDGT), hydroxylated isoprenoidal GDD (OH-GDD), overly branched GDGT (OB-GDGT), sparsely branched GDGT (SB-GDGT) and an abundant H-shaped GDGT with the [M+H]+ ion of m/z 1020 (H-1020). CONCLUSIONS: Characteristic MS/MS fragmentation patterns provided mass spectral 'fingerprints' for the recognition of diverse and prominent glycerol ether lipids. The ubiquitous distribution and substantial abundance of these glycerol ethers, as well as their structural variability, suggest a significant ecological role of their source organisms in various marine environments.

    Original languageEnglish
    Pages (from-to)2295-2302
    Number of pages8
    JournalRapid Communications in Mass Spectrometry
    Volume26
    Issue number19
    DOIs
    Publication statusPublished - 15 Oct 2012

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