Extensive genetic diversity in Iranian pomegranate (Punica granatum L.) germplasm revealed by microsatellite markers

Mehrbanoo Kazemi alamuti, Mehrshad Zeinalabedini, Mahsa Mousavi Derazmahalleh, Taha RoodbarShojaie, Hashem Poor Irandoost, Mehdi Zahravi, Mohammad Vazifehshenas, Mohammad Ali Ebrahimi, Sayed Mojtaba Khayam Nekouei, Ghasem Hossini Salekdeh, Mohsen Mardi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


A set of twelve simple sequence repeat markers was used to evaluate the genetic background of 738 pomegranate (Punica granatum L.) accessions, which belonged to the Iranian National Pomegranate Collection, from 23 provinces of Iran. Genetic diversity among the accessions was analysed using several variability parameters. Forty-three alleles were detected with a mean of 3.59 alleles per locus. The highest levels of polymorphism were found for the locus ABRII-MP26. The mean values of expected heterozygosity and polymorphic information content were 0.521 and 0.458, respectively. Neighbour-Net analysis based on uncorrected P distances and a model-based method separated the accessions into 8 main groups and several subgroups, with a tendency to cluster by geographical origin. From the simple sequence repeat analysis, it can be concluded that the genetic base of the Iranian National Pomegranate Collection is broad enough to ensure future progress in breeding programmes and these markers are effective tools for conducting genetic diversity studies in pomegranate and developing strategies for germplasm management.

Original languageEnglish
Pages (from-to)104-114
Number of pages11
JournalScientia Horticulturae
Publication statusPublished - 15 Oct 2012
Externally publishedYes


  • pomegranate
  • genetic variability
  • fluorescent
  • microsatellite
  • structure


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