TY - JOUR
T1 - Extinction coefficient for red-shifted chlorophylls
T2 - Chlorophyll d and chlorophyll f
AU - Li, Yaqiong
AU - Scales, Nicholas
AU - Blankenship, Robert E.
AU - Willows, Robert D.
AU - Chen, Min
PY - 2012/8
Y1 - 2012/8
N2 - Both chlorophyll f and chlorophyll d are red-shifted chlorophylls in oxygenic photosynthetic organisms, which extend photon absorbance into the near infrared region. This expands the range of light that can be used to drive photosynthesis. Quantitative determination of chlorophylls is a crucial step in the investigation of chlorophyll-photosynthetic reactions in the field of photobiology and photochemistry. No methods have yet been worked out for the quantitative determination of chlorophyll f. There is also no method available for the precise quantitative determination of chlorophyll d although it was discovered in 1943. In order to obtain the extinction coefficients (ε) of chlorophyll f and chlorophyll d, the concentrations of chlorophylls were determined by Inductive Coupled Plasma Mass Spectrometry according to the fact that each chlorophyll molecule contains one magnesium (Mg) atom. Molar extinction coefficient εchl f is 71.11 × 103 L mol- 1 A707 nm cm- 1 and εchl d is 63.68 × 103 L mol- 1 A697 nm cm - 1 in 100% methanol. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.
AB - Both chlorophyll f and chlorophyll d are red-shifted chlorophylls in oxygenic photosynthetic organisms, which extend photon absorbance into the near infrared region. This expands the range of light that can be used to drive photosynthesis. Quantitative determination of chlorophylls is a crucial step in the investigation of chlorophyll-photosynthetic reactions in the field of photobiology and photochemistry. No methods have yet been worked out for the quantitative determination of chlorophyll f. There is also no method available for the precise quantitative determination of chlorophyll d although it was discovered in 1943. In order to obtain the extinction coefficients (ε) of chlorophyll f and chlorophyll d, the concentrations of chlorophylls were determined by Inductive Coupled Plasma Mass Spectrometry according to the fact that each chlorophyll molecule contains one magnesium (Mg) atom. Molar extinction coefficient εchl f is 71.11 × 103 L mol- 1 A707 nm cm- 1 and εchl d is 63.68 × 103 L mol- 1 A697 nm cm - 1 in 100% methanol. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.
UR - http://www.scopus.com/inward/record.url?scp=84862806740&partnerID=8YFLogxK
U2 - 10.1016/j.bbabio.2012.02.026
DO - 10.1016/j.bbabio.2012.02.026
M3 - Article
C2 - 22395150
AN - SCOPUS:84862806740
SN - 0005-2728
VL - 1817
SP - 1292
EP - 1298
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 8
ER -