Extraction and stabilisation of the dimer of chlorophyll-protein complex II

Roger G. Hiller*, Theodora B G Pilger, Donna Campbell

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A dimer of chlorophyll-protein Complex II (CP II) can be separated in high yield by electrophoresis in sodium dodecylsulphate (SDS) polyacrylamide gels after solubilisation of chloroplast thylakoids in 1% Triton X-100 or Triton/S SDS mixtures. Its half life at 4°C is approx. 6 days. The dimer is readily converted to the monomer by addition of SDS to thylakoid extracts after solubilisation in Triton. Conversion of monomer to dimer by Triton addition to SDS solubilised extracts was not achieved.

Original languageEnglish
Pages (from-to)7-11
Number of pages5
JournalPlant Science Letters
Volume14
Issue number1
DOIs
Publication statusPublished - 1979

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