Extraction and stabilisation of the dimer of chlorophyll-protein complex II

Roger G. Hiller; Theodora B G Pilger; Donna Campbell

doi:10.1016/0304-4211(79)90148-2

Extraction and stabilisation of the dimer of chlorophyll-protein complex II

Roger G. Hiller^*, Theodora B G Pilger, Donna Campbell

^*Corresponding author for this work

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Abstract

A dimer of chlorophyll-protein Complex II (CP II) can be separated in high yield by electrophoresis in sodium dodecylsulphate (SDS) polyacrylamide gels after solubilisation of chloroplast thylakoids in 1% Triton X-100 or Triton/S SDS mixtures. Its half life at 4°C is approx. 6 days. The dimer is readily converted to the monomer by addition of SDS to thylakoid extracts after solubilisation in Triton. Conversion of monomer to dimer by Triton addition to SDS solubilised extracts was not achieved.

Original language	English
Pages (from-to)	7-11
Number of pages	5
Journal	Plant Science Letters
Volume	14
Issue number	1
DOIs	https://doi.org/10.1016/0304-4211(79)90148-2
Publication status	Published - 1979

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abstract = "A dimer of chlorophyll-protein Complex II (CP II) can be separated in high yield by electrophoresis in sodium dodecylsulphate (SDS) polyacrylamide gels after solubilisation of chloroplast thylakoids in 1% Triton X-100 or Triton/S SDS mixtures. Its half life at 4°C is approx. 6 days. The dimer is readily converted to the monomer by addition of SDS to thylakoid extracts after solubilisation in Triton. Conversion of monomer to dimer by Triton addition to SDS solubilised extracts was not achieved.",

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AU - Hiller, Roger G.

AU - Pilger, Theodora B G

AU - Campbell, Donna

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N2 - A dimer of chlorophyll-protein Complex II (CP II) can be separated in high yield by electrophoresis in sodium dodecylsulphate (SDS) polyacrylamide gels after solubilisation of chloroplast thylakoids in 1% Triton X-100 or Triton/S SDS mixtures. Its half life at 4°C is approx. 6 days. The dimer is readily converted to the monomer by addition of SDS to thylakoid extracts after solubilisation in Triton. Conversion of monomer to dimer by Triton addition to SDS solubilised extracts was not achieved.

AB - A dimer of chlorophyll-protein Complex II (CP II) can be separated in high yield by electrophoresis in sodium dodecylsulphate (SDS) polyacrylamide gels after solubilisation of chloroplast thylakoids in 1% Triton X-100 or Triton/S SDS mixtures. Its half life at 4°C is approx. 6 days. The dimer is readily converted to the monomer by addition of SDS to thylakoid extracts after solubilisation in Triton. Conversion of monomer to dimer by Triton addition to SDS solubilised extracts was not achieved.

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VL - 14

SP - 7

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JO - Plant Science Letters

JF - Plant Science Letters

IS - 1

ER -

Extraction and stabilisation of the dimer of chlorophyll-protein complex II

Abstract

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