Extraction, isolation and purification of exopolysaccharide from lactic acid bacteria using ethanol precipitation method

Vivek K. Bajpai; Rajib Majumder; Irfan A. Rather; Kangmin Kim

doi:10.3329/bjp.v11i3.27170

Extraction, isolation and purification of exopolysaccharide from lactic acid bacteria using ethanol precipitation method

Vivek K. Bajpai^*, Rajib Majumder, Irfan A. Rather, Kangmin Kim

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

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Abstract

Lactic acid bacteria are classified ‘Generally Recognized As Safe’ (GRAS) with most effective potential to divert significant amount of fermentable sugars towards the biosynthesis of functional exopolysaccharide. Exopolysaccharides from lactic acid bacteria are receiving a renewed interest due to the claims of human health benefits, such as modulation of immune response system and more importantly in food and pharma industries as a texturizer, viscosifer, emulsifier and syneresis-lowering agent. Its purification methodology involves: a) Extraction of cell-free supernatant from lactic acid bacteria; b) Denature of protein using trichloroacetic acid; c) Ethanol precipitation; d) Dialysis; and e) Freeze drying. However, depending on nature of research, compounds can be further purified using scanning electron microscopy (SEM), infrared spectrum (IR); and nuclear magnetic resonance (NMR) spectral analyses.

Original language	English
Pages (from-to)	573-576
Number of pages	4
Journal	Bangladesh Journal of Pharmacology
Volume	11
Issue number	3
DOIs	https://doi.org/10.3329/bjp.v11i3.27170
Publication status	Published - 2 Jun 2016
Externally published	Yes

Bibliographical note

Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

Exopolysaccharide
Lactic acid bacteria

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10.3329/bjp.v11i3.27170

Publisher versionFinal published version, 341 KBLicence: CC BY

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title = "Extraction, isolation and purification of exopolysaccharide from lactic acid bacteria using ethanol precipitation method",

abstract = "Lactic acid bacteria are classified {\textquoteleft}Generally Recognized As Safe{\textquoteright} (GRAS) with most effective potential to divert significant amount of fermentable sugars towards the biosynthesis of functional exopolysaccharide. Exopolysaccharides from lactic acid bacteria are receiving a renewed interest due to the claims of human health benefits, such as modulation of immune response system and more importantly in food and pharma industries as a texturizer, viscosifer, emulsifier and syneresis-lowering agent. Its purification methodology involves: a) Extraction of cell-free supernatant from lactic acid bacteria; b) Denature of protein using trichloroacetic acid; c) Ethanol precipitation; d) Dialysis; and e) Freeze drying. However, depending on nature of research, compounds can be further purified using scanning electron microscopy (SEM), infrared spectrum (IR); and nuclear magnetic resonance (NMR) spectral analyses.",

keywords = "Exopolysaccharide, Lactic acid bacteria",

author = "Bajpai, {Vivek K.} and Rajib Majumder and Rather, {Irfan A.} and Kangmin Kim",

note = "Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.",

year = "2016",

month = jun,

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doi = "10.3329/bjp.v11i3.27170",

language = "English",

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pages = "573--576",

journal = "Bangladesh Journal of Pharmacology",

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TY - JOUR

T1 - Extraction, isolation and purification of exopolysaccharide from lactic acid bacteria using ethanol precipitation method

AU - Bajpai, Vivek K.

AU - Majumder, Rajib

AU - Rather, Irfan A.

AU - Kim, Kangmin

N1 - Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2016/6/2

Y1 - 2016/6/2

N2 - Lactic acid bacteria are classified ‘Generally Recognized As Safe’ (GRAS) with most effective potential to divert significant amount of fermentable sugars towards the biosynthesis of functional exopolysaccharide. Exopolysaccharides from lactic acid bacteria are receiving a renewed interest due to the claims of human health benefits, such as modulation of immune response system and more importantly in food and pharma industries as a texturizer, viscosifer, emulsifier and syneresis-lowering agent. Its purification methodology involves: a) Extraction of cell-free supernatant from lactic acid bacteria; b) Denature of protein using trichloroacetic acid; c) Ethanol precipitation; d) Dialysis; and e) Freeze drying. However, depending on nature of research, compounds can be further purified using scanning electron microscopy (SEM), infrared spectrum (IR); and nuclear magnetic resonance (NMR) spectral analyses.

AB - Lactic acid bacteria are classified ‘Generally Recognized As Safe’ (GRAS) with most effective potential to divert significant amount of fermentable sugars towards the biosynthesis of functional exopolysaccharide. Exopolysaccharides from lactic acid bacteria are receiving a renewed interest due to the claims of human health benefits, such as modulation of immune response system and more importantly in food and pharma industries as a texturizer, viscosifer, emulsifier and syneresis-lowering agent. Its purification methodology involves: a) Extraction of cell-free supernatant from lactic acid bacteria; b) Denature of protein using trichloroacetic acid; c) Ethanol precipitation; d) Dialysis; and e) Freeze drying. However, depending on nature of research, compounds can be further purified using scanning electron microscopy (SEM), infrared spectrum (IR); and nuclear magnetic resonance (NMR) spectral analyses.

KW - Exopolysaccharide

KW - Lactic acid bacteria

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DO - 10.3329/bjp.v11i3.27170

M3 - Article

AN - SCOPUS:84973513835

SN - 1991-007X

VL - 11

SP - 573

EP - 576

JO - Bangladesh Journal of Pharmacology

JF - Bangladesh Journal of Pharmacology

IS - 3

ER -

Extraction, isolation and purification of exopolysaccharide from lactic acid bacteria using ethanol precipitation method

Abstract

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Keywords

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