TY - JOUR
T1 - Extraction of DNA from acidic, hydrothermally modified volcanic soils
AU - Henneberger, Ruth M.
AU - Walter, Malcolm R.
AU - Anitori, Roberto P.
PY - 2006
Y1 - 2006
N2 - Acidic soils for microbial diversity studies were collected from Devil's Kitchen, a fumarolic field on Mt Hood, USA. The very dense soils, which contain clay and other minerals, are derived from andesitic and dacitic rocks altered by volcanic heat and acidic, sulfur-rich hydrothermal steam. An initial attempt to extract biomass DNA using a mechanical-based cell lysis protocol was ineffective. However, by using various other protocols, DNA was successfully extracted, leading to the identification of several acidophilic Mt Hood extremophiles. The results emphasise the importance of testing different extraction procedures when dealing with apparently intractable samples.
AB - Acidic soils for microbial diversity studies were collected from Devil's Kitchen, a fumarolic field on Mt Hood, USA. The very dense soils, which contain clay and other minerals, are derived from andesitic and dacitic rocks altered by volcanic heat and acidic, sulfur-rich hydrothermal steam. An initial attempt to extract biomass DNA using a mechanical-based cell lysis protocol was ineffective. However, by using various other protocols, DNA was successfully extracted, leading to the identification of several acidophilic Mt Hood extremophiles. The results emphasise the importance of testing different extraction procedures when dealing with apparently intractable samples.
UR - http://www.scopus.com/inward/record.url?scp=33646505082&partnerID=8YFLogxK
U2 - 10.1071/EN06013
DO - 10.1071/EN06013
M3 - Article
AN - SCOPUS:33646505082
SN - 1448-2517
VL - 3
SP - 100
EP - 104
JO - Environmental Chemistry
JF - Environmental Chemistry
IS - 2
ER -